Soy isoflavone supplementation in healthy men prevents NF-κB activation by TNF-α in blood lymphocytes
Soy isoflavone supplementation in healthy men prevents NF-κB activation by TNF-α in blood lymphocytes
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DOI：
10.1016/s0891-5849(01)00535-4
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发表时间：
2001-06-01
影响因子：
7.4
通讯作者：
Sarkar, FH
中科院分区：
文献类型：
--
作者：
Davis, JN;Kucuk, O;Sarkar, FH
饮食中摄入大豆与降低癌症风险有关。大豆异黄酮被认为是大豆中的保护性化合物；然而，大豆异黄酮预防人类癌症的确切机制尚不清楚。主要的大豆异黄酮、染料木黄酮和大豆黄酮是抗氧化化合物，因此一种可能的作用机制是通过它们的抗氧化作用。我们之前已经证明，大豆异黄酮、金雀异黄酮可在体外抑制前列腺癌细胞中氧化还原敏感转录因子 NF-κB 的激活。在这项研究中，我们证明金雀异黄酮（而非大豆黄酮）可抑制培养的人淋巴细胞中 TNF-α 诱导的 NF-κB 活化。此外，我们还研究了补充大豆异黄酮对六名健康男性体外外周血淋巴细胞中 TNF-α 诱导的 NF-κB 激活的体内影响。我们发现，健康男性受试者每天两次服用 50 毫克异黄酮混合物（Novasoy），持续 3 周，可免受 TNF-α 诱导的 NF-κB 激活。此外，我们观察到补充异黄酮后，5-羟甲基-2'-脱氧尿苷 (5-OHmdU) 减少，这是氧化 DNA 损伤的标志物。补充3个月后，大豆异黄酮的抑制作用不再存在。这项初步研究表明，补充大豆异黄酮可以通过抑制 NF-kappaB 激活和降低 DNA 加合物水平来保护细胞免受氧化应激诱导剂的侵害。 (C) 2001 爱思唯尔科学公司。
Dietary intake of soy has been associated with a decreased risk of cancer. Soy isoflavones have been postulated to be the protective compounds in soybeans; however, the precise mechanism by which soy isoflavones prevent human cancer is not known. The major soy isoflavones, genistein and daidzein, are antioxidant compounds, therefore one possible mechanism of action is through their antioxidant effect. We have previously demonstrated that the soy isoflavone, genistein, inhibits the activation of the redox-sensitive transcription factor, NF-kappaB, in prostate cancer cells in vitro. In this study, we have demonstrated that genistein, but not daidzein, inhibits TNF-alpha -induced NF-kappaB activation in cultured human lymphocytes. Additionally, we investigated the in vivo effect of soy isoflavone supplementation on NF-kappaB activation induced by TNF-alpha in vitro in peripheral blood lymphocytes of six healthy men. We show that healthy male subjects receiving 50 mg isoflavone mixture (Novasoy) twice daily for 3 weeks are protected from TNF-alpha induced NF-kappaB activation. Additionally, we observed a reduction of 5-hydroxymethyl-2' -deoxyuridine (5-OHmdU), a marker for oxidative DNA damage, following isoflavone supplementation. The inhibitory effect of soy isoflavones was no longer present 3 months after the supplementation. This preliminary study demonstrates that soy isoflavone supplementation may protect cells from oxidative stress-inducing agents by inhibiting NF-kappaB activation and decreasing DNA adduct levels. (C) 2001 Elsevier Science Inc.