Fluorometric competitive immunoassay for chlorpyrifos using rhodamine-modified gold nanoparticles as a label
Fluorometric competitive immunoassay for chlorpyrifos using rhodamine-modified gold nanoparticles as a label
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以罗丹明修饰金纳米颗粒为标记的毒死蜱荧光竞争免疫分析
DOI：
10.1007/s00604-017-2561-0
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发表时间：
2018-01-01
影响因子：
5.7
通讯作者：
Yang, Meihua
中科院分区：
文献类型：
--
作者：
Dou, Xiaowen;Zhang, Lei;Yang, Meihua
以罗丹明B异硫氰酸盐（RBITC）为荧光基团，通过修饰金纳米粒子（AuNPs），制备了一种新型的标记抗体。将由此获得的缀合物用于具有开启信号变化的竞争性夹心测定。该方法用于有机磷农药毒死蜱的检测。RBITC标记的金免疫探针的荧光在575 nm处发射，在556 nm处激发，几乎完全猝灭。然而，如果添加半胱胺，则荧光团从标记的二抗释放，并且荧光根据结合至夹心的二抗的量而增加。应用该方法测定了干陈皮中毒死蜱的含量。检测结果也通过LC-MS/MS进行了独立确认。该方法可将毒死蜱的浓度定量至4.9 ng·mL−1，相当于干果皮中的61 μg·kg− 1。在我们看来，这种方法具有广泛的潜力，可应用于许多分析物的抗体available.Graphical abstractA种新的标记抗体的合成修饰金纳米粒子与荧光团罗丹明B异硫氰酸酯（RBITC）和第二抗体（IgG）。该缀合物用于荧光免疫测定，具有开启信号变化。建立了有机磷农药毒死蜱的检测方法。
A new kind of labelled antibody was synthesized by modifying gold nanoparticles (AuNPs) with the fluorophore rhodamine B isothiocyanate (RBITC) and a secondary antibody (IgG). The conjugate thus obtained was used in a competitive sandwich assay with a turn-on signal change. It was designed to detect the organophosphorus pesticide chlorpyrifos. The fluorescence of the RBITC-labeled gold immunoprobe with emission at 575 nm and excitation at 556 nm is almost completely quenched. If, however, cysteamine is added, the fluorophore is released from the labeled secondary antibody and fluorescence increases in accordance with the quantity of secondary antibody bound to the sandwich. This assay was applied to determine chlorpyrifos in dried tangerine peels. The detection results were also independently confirmed by LC-MS/MS. The method allows the concentrations of chlorpyrifos to be quantified down to 4.9 ng·mL−1, which is equivalent to 61 μg·kg−1in dried tangerine peels. In our perception, this approach has a wide potential to be applied in the determination of numerous analytes for which antibodies are available.Graphical abstractA new kind of labelled antibody was synthesized by modifying gold nanoparticles with fluorophore rhodamine B isothiocyanate (RBITC) and a secondary antibody (IgG). The conjugate was used in fluoroimmunoassay with a turn-on signal change. The method was designed to detect the organophosphorus pesticide chlorpyrifos.