CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS
CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS
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DOI：
10.1007/bf02558256
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发表时间：
1965-01-01
影响因子：
2
通讯作者：
WHITE, JL
中科院分区：
文献类型：
--
作者：
SINGLETON, WS;GRAY, MS;WHITE, JL
通过对现有的、冗长的方法进行改进，用氧化铝柱层析法从粗鸡蛋磷脂中分离出了层析均匀的鸡蛋卵磷脂，通过硅胶- g薄层析（TLC）测定。改进后的方法是将粗鸡蛋磷脂按1g磷脂/ 25g氧化铝的比例加入到氧化铝柱中，用2组分溶剂体系氯仿甲醇（体积比为9:1）洗脱卵磷脂部分。该纯化方法将卵磷脂与粗磷脂中的其他胆碱和非胆碱成分分离，避免了氧化铝柱的过载。并且不需要对部分纯化的卵磷脂在硅酸上进行第二次色谱分离，而在现有的卵磷脂纯化方法中需要进行第二次色谱分离。使用新鲜蛋黄比使用商业干燥的蛋黄更容易从最终产品中去除色素。从干蛋黄中提取的磷脂比从新鲜蛋黄中提取的磷脂更显色，并且通过氧化铝层析可以保持颜色。所得卵磷脂的脂肪酸/磷摩尔比为2.00，为磷脂酰胆碱的理论FA/P摩尔比；其他没有这个比例的材料。
Chromatographically homogeneous egg lecithin, as determined by thin layer chromatography (TLC) on Silica Gel-G, has been isolated from crude egg phosphatides by column chromatography on alumina through modification of existing, lengthy methods. The modified method involved application of crude egg phosphatides to a column of alumina in the proportion of 1 g phosphatide/25 g alumina, and elution of the lecithin fraction with the 2-component solvent system chloroform methanol, 9:1 by vol. This method of purification separated lecithin from other choline and non-choline components of crude phosphatides, avoided overloading of the alumina column, and made unnecessary the need for a second chromatographic fractionation of partially purified lecithin on silicic acid, which is needed in existing methods of purification of lecithin. The use of fresh yolks permitted easier removal of pigment from the final product than was possible with commercially dried yolks. Phosphatides extracted from dried yolks were much more highly colored than were the phosphatides extracted from fresh yolks and the color persisted through chromatography on alumina. The fatty-acid/phosphorus molar ratio of the purified lecithin was 2.00, which is the theoretical FA/P molar ratio of phosphatidylcholine; other materials with this ratio were not present.