MICRO-CALORIMETRIC STUDY OF ESCHERICHIA-COLI AEROBIC GROWTH - THEORETICAL ASPECTS OF GROWTH ON SUCCINIC ACID
MICRO-CALORIMETRIC STUDY OF ESCHERICHIA-COLI AEROBIC GROWTH - THEORETICAL ASPECTS OF GROWTH ON SUCCINIC ACID
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DOI：
10.1128/jb.143.2.742-746.1980
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发表时间：
1980-01-01
影响因子：
3.2
通讯作者：
BELAICH, JP
中科院分区：
文献类型：
--
作者：
DERMOUN, Z;BELAICH, JP
本文采用两种研究方法对好氧生长过程中与合成代谢过程有关的热量进行了测定。大肠杆菌在含有琥珀酸作为唯一能量和C源的基本培养基中进行。用这两种方法研究了琥珀酸和NH3对生物合成反应的贡献。获得的2个qan值非常一致，具有显著性。因此，这表明，厌氧微生物的贡献强烈影响与微生物需氧生长相关的热量。用上述方法计算的qan解释了最近报道的实验焓变。
Two methods of investigation were used to evaluate the heat quantity associated with anabolic processes (qan) during the aerobic growth of E. coli in a minimal medium containing succinic acid as the sole energy and C source. The study of the contribution of biosynthetic reactions from succinic acid and NH3 were investigated by both methods. The 2 qan values obtained were in excellent agreement and were significant. Thus it was demonstrated that the contribution of anabolism strongly influenced the quantity of heat associated with microbial aerobic growth. The qan calculated as above explained the experimental enthalpy change which was recently reported.