Sequestration of polyunsaturated fatty acids in membrane phospholipids of Caenorhabditis elegans dauer larva attenuates eicosanoid biosynthesis for prolonged survival.
Sequestration of polyunsaturated fatty acids in membrane phospholipids of Caenorhabditis elegans dauer larva attenuates eicosanoid biosynthesis for prolonged survival.
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秀丽隐杆线虫幼虫膜磷脂中多不饱和脂肪酸的隔离会减弱类二十烷酸的生物合成，从而延长存活时间
DOI：
10.1016/j.redox.2017.05.002
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发表时间：
2017-08
期刊：
影响因子：
11.4
通讯作者：
Shui G
中科院分区：
文献类型：
--
作者：
Lam SM;Wang Z;Li J;Huang X;Shui G
机械基础管理的极端长寿和发展的静止的dauer少年，一个“非老化”的发展变化的秀丽隐杆线虫，在很大程度上仍然是模糊的。使用lipidomic方法，包括多个反应监测特定于不同的脂肪酰基部分的过渡，我们表明，在其他发育阶段相比，dauer幼虫的膜磷脂含有一个独特的富集的多不饱和脂肪酸（PUFA）。磷脂中的酯化PUFA在整个滞育耐受过程中表现出暂时积累，然后在滞育终止前急剧减少。酯化PUFA的减少伴随着未结合PUFA及其相应下游氧化衍生物（即类二十烷酸）的伴随增加。全球磷脂组学已经揭示，膜磷脂中的PUFA螯合表示dauer休眠的一个重要方面，主要是通过抑制类花生酸的产生;并且未能维持膜脂质稳态与dauer耐力的终止相关。与其他幼虫阶段相比，Dauer在磷脂中含有更多的PUFA。在滞育结束前，整个滞育期内PUFA的积累都丧失了。在滞育结束之前，类花生酸的产生显著增加。膜脂动态的变化可能是dauer滞育机制的基础。
Mechanistic basis governing the extreme longevity and developmental quiescence of dauer juvenile, a “non-ageing” developmental variant of Caenorhabditis elegans, has remained largely obscure. Using a lipidomic approach comprising multiple reaction monitoring transitions specific to distinct fatty acyl moieties, we demonstrated that in comparison to other developmental stages, the membrane phospholipids of dauer larva contain a unique enrichment of polyunsaturated fatty acids (PUFAs). Esterified PUFAs in phospholipids exhibited temporal accumulation throughout the course of dauer endurance, followed by sharp reductions prior to termination of diapause. Reductions in esterified PUFAs were accompanied by concomitant increases in unbound PUFAs, as well as their corresponding downstream oxidized derivatives (i.e. eicosanoids). Global phospholipidomics has unveiled that PUFA sequestration in membrane phospholipids denotes an essential aspect of dauer dormancy, principally via suppression of eicosanoid production; and a failure to upkeep membrane lipid homeostasis is associated with termination of dauer endurance. Dauer contains more PUFAs in phospholipids compared to other larval stages. PUFA accumulation throughout dauer endurance is lost prior to the end of diapause. Marked increases in eicosanoid production precede the termination of diapause. Changes in membrane lipid dynamics possibly underlie mechanisms of dauer diapause.