Enhancement of Endothelialisation of Coronary Stents by Laser Surface Engineering
Enhancement of Endothelialisation of Coronary Stents by Laser Surface Engineering
复制标题
DOI：
10.1002/lsm.22180
复制
发表时间：
2013-11-01
影响因子：
2.4
通讯作者：
Wang, Tao
中科院分区：
文献类型：
--
作者：
Li, Lin;Mirhosseini, Nazanin;Wang, Tao
冠状动脉支架已广泛应用于冠心病的治疗。然而，并发症阻碍了该设备的长期成功。裸金属支架（BMS）具有较高的再狭窄率和较差的内皮化。药物洗脱支架（DES），虽然显着减少再狭窄，显着防止内皮化导致晚期血栓形成，并表现出相同的方式作为BMS药物释放后。内皮细胞在支架表面的快速粘附和生长是冠状动脉支架术后早期血管愈合的关键过程，有助于减少主要并发症。表面特性控制细胞生长，并直接决定植入物的成功和寿命。然而，冠状动脉支架的理想表面性质尚未完全了解。本研究的目的是了解由激光束产生的表面微/纳米纹理和相关材料化学变化如何影响裸金属316L Stents.Materials和MethodsA高功率激光束被施加到316L冠状动脉支架材料和商业冠状动脉支架的表面性质改性，接着检查在表面上生长的人冠状动脉内皮细胞的粘附和增殖。通过扫描电子显微镜，接触角测量，和X-射线光电子能谱（XPS）检查表面特性。ResultsA新的表面与组合的微/纳米功能的支架材料316 L和冠状动脉支架上创建一个特定的表面化学。该表面引起内皮细胞的粘附增加三倍，增殖增加八倍。有趣的是，只有在氮气气氛中产生表面纹理时才观察到这种效应，这表明表面化学的重要性，包括氮化铬的急剧增加，内皮细胞与材料表面的相互作用。这种新的表面也是超亲水性的接近零水/细胞培养液接触角和低cytotoxic.ConclusionsA新的表面激光表面工程与定义的表面纹理和表面化学相结合创建的冠状动脉支架内皮化的改善被发现是有益的。本文介绍的技术可以与DES和BMS一起使用，并为改善当前冠状动脉支架的生物相容性带来额外的益处。激光外科医学45：608-616，2013年。(c)2013 Wiley Periodicals，Inc.
Background and ObjectiveCoronary stents have been widely used in the treatment of coronary heart disease. However, complications have hampered the long-term success of the device. Bare-metal stents (BMS) have a high rate of restenosis and poor endothelialisation. The drug-eluting stents (DES), although dramatically reduce restenosis, significantly prevent endothelialisation leading to late thrombosis and behave the same way as BMS after drug releasing. Rapid adhesion and growth of endothelial cells on the stent surface is a key process for early vascular healing after coronary stenting which contributes to the reduction of major complications. Surface properties manipulate cell growth and directly determine the success and life-span of the implants. However, the ideal surface properties of coronary stents are not yet fully understood. The objective of this research is to understand how surface micro/nano textures and associated material chemistry changes generated by a laser beam affect the behavior of endothelial cells on bare metal 316L stents.Materials and MethodsA high power laser beam was applied to modifying the surface properties of 316L coronary stent material and the commercial coronary stents, followed by examination of the adhesion and proliferation of human coronary endothelial cells that were growing on the surfaces. Surface properties were examined by scanning electron microscopy, contact angle measurement, and X-ray photoelectron spectroscopy.ResultsA novel surface with combined micro/nano features was created on stent material 316L and coronary stent with a specific surface chemistry. This surface gives rise to a threefold increase in the adhesion and eightfold increase in the proliferation of endothelial cells. Interestingly, such effects were only observed when the surface texture was produced in the nitrogen atmosphere suggesting the importance of the surface chemistry, including the dramatic increase of chromium nitride, for the interaction of endothelial cells with the material surface. This novel surface is also super-hydrophilic with close to zero water/cell culture fluid contact angles and low cytotoxicity.ConclusionsA novel surface created by laser surface-engineering with a combination of defined surface texture and surface chemistry was found beneficial for the improvement of coronary stent endothelialisation. The technology presented here could work with both DES and BMS with added benefit for the improvement of the biocompatibility of current coronary stents. Lasers Surg. Med. 45:608-616, 2013. (c) 2013 Wiley Periodicals, Inc.