Influence of vanadate on migrating fibroblast orientation within a fibrin matrix
Influence of vanadate on migrating fibroblast orientation within a fibrin matrix
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DOI：
10.1002/jcp.21475
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发表时间：
2008-10-01
影响因子：
5.6
通讯作者：
Ehrlich, H. Paul
中科院分区：
文献类型：
--
作者：
Lee, Michael Y.;Ehrlich, H. Paul
用非特异性蛋白酪氨酸磷酸酶抑制剂钒酸盐处理大鼠伤口肉芽组织中胶原纤维束的均匀包装，使大鼠切口伤口的伤口断裂强度加倍。推测是钒酸盐通过在填充缺陷的纤维蛋白凝块中新到达的伤口成纤维细胞的定向来优化胶原纤维束的包装。将14日龄鸡胚肌腱段置于纤维蛋白凝块上，在添加和不添加30 μ M钒酸盐的器官培养中维持。第7天对外植体进行组织学和生化检查。未经处理的外植体的肌腱成纤维细胞以随机方式迁移，而钒酸盐处理的外植体的成纤维细胞以线性阵列迁移。与对照组相比，钒酸盐处理的外植体的成纤维细胞延长了20%。肌凝蛋白atp酶是最佳细胞运动所必需的，通过其肌凝蛋白轻链（MLC）的磷酸化进行优化。对迁移到纤维蛋白中的成纤维细胞的裂解物进行Western blot分析显示，钒酸盐增加了MLC-P水平。这些发现支持了这样一种观点，即在伤口修复过程的早期，钒酸盐通过促进成纤维细胞平行线性排列的方向，促进了规则平行胶原纤维束的沉积。
Treating rats with vanadate, a nonspecific inhibitor of protein tyrosine phosphatases, optimizes the uniform packing of collagen fiber bundles in wound granulation tissue and doubles wound breaking strength in rat incisional wounds. The speculation is vanadate optimizes the packing of collagen fiber bundles through the orientation of newly arrived wound fibroblasts in the fibrin clot filling the defect. Segments of 14 day chick embryo tendons were placed on fibrin clots and maintained in organ culture with and without 30 mu M vanadate. On day 7 explants were examined histologically and biochemically. Tendon fibroblast outgrowth from untreated explants migrated in a random fashion, while fibroblasts from vanadate-treated explants migrated out in linear arrays. Fibroblasts were elongated by 20% form vanadate treated explant compared to controls. Myosin ATPase, required for optimal cell motility, is optimized by the phosphorylation of its myosin light chain (MLC). Western blot analysis of lysates from the fibroblasts that migrated into the fibrin showed vanadate increased MLC-P levels. These findings support the notion that vanadate promotes the deposition of regular, parallel collagen fiber bundles by advancing the orientation of fibroblasts in parallel linear arrays early in the wound repair process.