A phosphoproteomic landscape of rice (Oryza sativa) tissues
A phosphoproteomic landscape of rice (Oryza sativa) tissues
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水稻（Oryza sativa）组织的磷酸化蛋白质组学景观
DOI：
10.1111/ppl.12574
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发表时间：
2017-08-01
影响因子：
6.4
通讯作者：
Zhang，Jian
中科院分区：
文献类型：
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作者：
Wang，Yifeng;Tong，Xiaohong;Zhang，Jian
蛋白质磷酸化是一种重要的翻译后修饰，调控着植物的多种发育过程。在这里，我们报告了一个全面的，定量的磷蛋白质组学分析，包括愈伤组织，叶，根，茎分生组织，年轻穗和成熟穗从Nipponbare利用质谱（MS）为基础的，无标签的方法。在2657个磷酸化蛋白的4792个磷酸肽中，共鉴定出7171个独特的磷酸化位点，其中4613个肽在组织中存在差异磷酸化（DP）。Motif‐X分析显示8个显著富集的基序，如[sP]、[Rxxs]和[tP]。分层聚类分析将DP肽分为63个亚群，这些亚群在不同组织中表现出不同的空间磷酸化模式。这些聚集蛋白在功能上与根的营养吸收、叶片的光合作用和穗的组织分化有关。磷酸化在靶蛋白执行其功能的组织中是特异性的，这表明磷酸化可能是调节不同组织中蛋白质活性的关键机制。该研究极大地扩展了水稻磷酸化蛋白质组学数据集，并深入了解了磷酸化在组织发育和功能中的调节作用。
Protein phosphorylation is an important posttranslational modification that regulates various plant developmental processes. Here, we report a comprehensive, quantitative phosphoproteomic profile of six rice tissues, including callus, leaf, root, shoot meristem, young panicle and mature panicle from Nipponbare by employing a mass spectrometry (MS)‐based, label‐free approach. A total of 7171 unique phosphorylation sites in 4792 phosphopeptides from 2657 phosphoproteins were identified, of which 4613 peptides were differentially phosphorylated (DP) among the tissues. Motif‐X analysis revealed eight significantly enriched motifs, such as [sP], [Rxxs] and [tP] from the rice phosphosites. Hierarchical clustering analysis divided the DP peptides into 63 subgroups, which showed divergent spatial‐phosphorylation patterns among tissues. These clustered proteins are functionally related to nutrition uptake in roots, photosynthesis in leaves and tissue differentiation in panicles. Phosphorylations were specific in the tissues where the target proteins execute their functions, suggesting that phosphorylation might be a key mechanism to regulate the protein activity in different tissues. This study greatly expands the rice phosphoproteomic dataset, and also offers insight into the regulatory roles of phosphorylation in tissue development and functions.