Molecular regulation of santalol biosynthesis in Santalum album L.
Molecular regulation of santalol biosynthesis in Santalum album L.
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DOI：
10.1016/j.gene.2013.06.080
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发表时间：
2013-09-25
期刊：
影响因子：
3.5
通讯作者：
Kush, Anil
中科院分区：
文献类型：
--
作者：
Rani, Arti;Ravikumar, Puja;Kush, Anil
檀香（Santalum album L），俗称东印度檀香（East-Indian sandal）或香丹（Chandan），是檀香科的一种半寄生树。檀香醇是存在于檀香中的生物勘探分子，并且对这一重要部分的代谢工程的任何努力都需要基因调控方面的知识。檀香醇是通过甲羟戊酸或非甲羟戊酸途径合成的倍半萜。檀香醇生物合成的第一步涉及异戊烯基焦磷酸（IPP）与其烯丙基共底物二甲基烯丙基焦磷酸（DMAPP）的首尾缩合以产生香叶基焦磷酸（GPP; C10 -单萜）。GPP在与IPP的一次额外缩合后产生焦磷酸法呢酯（FPP; C15 -开链倍半萜）。这两个反应都是由法呢基二磷酸合酶（FDS）催化的。檀香烯合酶（SS）是一种萜烯环化酶，其催化开环FPP环化为环状倍半萜的混合物，例如檀香烯、表-β-檀香烯、β-檀香烯和外香柠檬烯（檀香油的主要成分）。本工作的目的是产生一个全面的知识，涉及檀香醇生产的基因，并研究其分子调控。为了实现这一点，法呢基二磷酸合成酶和檀香烯合成酶的编码序列分离檀香木使用抑制消减杂交和二维凝胶电泳技术。这两个基因的功能特性，通过酶试验和组织特异性表达的两个基因进行了研究。据我们所知，这是第一个研究檀香醇生物合成的分子调控和组织特异性表达的基因的报告。(C)2013 Elsevier B. V.保留所有权利。
Santalum album L commonly known as East-Indian sandal or chandan is a hemiparasitic tree of family santalaceae. Santalol is a bioprospecting molecule present in sandalwood and any effort towards metabolic engineering of this important moiety would require knowledge on gene regulation. Santalol is a sesquiterpene synthesized through mevalonate or non-mevalonate pathways. First step of santalol biosynthesis involves head to tail condensation of isopentenyl pyrophosphate (IPP) with its allylic co-substrate dimethyl allyl pyrophosphate (DMAPP) to produce geranyl pyrophosphate (GPP; C10 - a monoterpene). GPP upon one additional condensation with IPP produces farnesyl pyrophosphate (FPP; C15 - an open chain sesquiterpene). Both the reactions are catalyzed by famesyl diphosphate synthase (FDS). Santalene synthase (SS), a terpene cyclase catalyzes cyclization of open ring FPP into a mixture of cyclic sesquiterpenes such as alpha-santalene, epi-beta-santalene, beta-santalene and exo bergamotene, the main constituents of sandal oil. The objective of the present work was to generate a comprehensive knowledge on the genes involved in santalol production and study their molecular regulation. To achieve this, sequences encoding famesyl diphosphate synthase and santalene synthase were isolated from sandalwood using suppression subtraction hybridization and 2D gel electrophoresis technology. Functional characterization of both the genes was done through enzyme assays and tissue-specific expression of both the genes was studied. To our knowledge, this is the first report on studies on molecular regulation, and tissue-specific expression of the genes involved in santalol biosynthesis. (C) 2013 Elsevier B.V. All rights reserved.