Transcription inhibition using oligonucleotide-modified gold nanoparticles
Transcription inhibition using oligonucleotide-modified gold nanoparticles
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DOI：
10.1021/bc060100f
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发表时间：
2006-09-20
影响因子：
4.7
通讯作者：
Feldheim, Daniel
中科院分区：
文献类型：
--
作者：
Agbasi-Porter, Chiamaka;Ryman-Rasmussen, Jessica;Feldheim, Daniel
已经证明了使用金纳米颗粒表面上的双链启动子DNA捕获T7 RNA聚合酶。由于在纳米颗粒表面上的特异性相互作用，T7 RNA聚合酶的竞争性结合和抑制代表了模型系统中的转录因子诱饵方法。针对金纳米颗粒上的双链启动子DNA的各种纳米颗粒尺寸、表面覆盖率和接头长度确定抑制效率。这些实验为确定纳米颗粒表面上的结合位点的可及性提供了基础，用于涉及细胞靶向或使用纳米颗粒作为溶液中的结合剂的应用。
The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution.