Mesenchymal-Like Stem Cells Derived from Human Parthenogenetic Embryonic Stem Cells
Mesenchymal-Like Stem Cells Derived from Human Parthenogenetic Embryonic Stem Cells
复制标题
源自人类孤雌胚胎干细胞的间充质样干细胞
DOI：
10.1089/scd.2010.0585
复制
发表时间：
2012-01-01
影响因子：
4
通讯作者：
Zhang, Wen Jie
中科院分区：
文献类型：
--
作者：
Chen, Yao;Ai, Ai;Zhang, Wen Jie
人孤雌胚胎干细胞（hpESCs）是由人工激活的卵母细胞建立的，由于其主要组织相容性复合体（MHC）基因的纯合性，具有广泛的免疫匹配能力。这些细胞是否具有类似于来自受精卵的常规人类胚胎干细胞（hESC）的分化能力尚不清楚。本研究的目的是确定hpESCs是否可以在体外分化为多能间充质干细胞（MSC）样细胞，然后比较这些细胞与那些来自hESCs。从hpESC和hESC获得MSC样细胞，其表现出相似的细胞表面标志物表达谱。进一步的分析表明，与hESC来源的细胞相比，hpESC来源的细胞具有更强的成骨能力，但较弱的成脂能力。这是第一个证明hpESC分化为多能MSC样细胞的工作。这些hpESCs可能是基于细胞的治疗的潜在来源。
Human parthenogenetic embryonic stem cells (hpESCs) established from artificially activated oocytes have a wider immune-matching ability because of their homozygosity in the major histocompatibility complex alleles. Whether these cells possess the differentiation capacity similar to regular human embryonic stem cells (hESCs) derived from fertilized eggs is unclear. The aims of this study were to determine whether hpESCs could be differentiated into multipotent mesenchymal stem cell (MSC)-like cells in vitro and then compare these cells with those derived from hESCs. MSC-like cells were obtained from both hpESCs and hESCs, which exhibited similar cell surface marker expression profiles. Further analyses revealed that cells derived from hpESCs possessed stronger osteogenic but weaker adipogenic potentials compared with cells derived from hESCs. This is the first work that demonstrates the differentiation of hpESCs into multipotent MSC-like cells. These hpESCs could be a potential source for cell-based therapies.