Convergent Loss of an EDS1/PAD4 Signaling Pathway in Several Plant Lineages Reveals Coevolved Components of Plant Immunity and Drought Response
Convergent Loss of an EDS1/PAD4 Signaling Pathway in Several Plant Lineages Reveals Coevolved Components of Plant Immunity and Drought Response
复制标题
DOI：
10.1105/tpc.19.00903
复制
发表时间：
2020-07-01
期刊：
影响因子：
11.6
通讯作者：
Krasileva, Ksenia, V
中科院分区：
文献类型：
--
作者：
Baggs, Erin L.;Monroe, J. Grey;Krasileva, Ksenia, V
基因组分析显示，一些植物失去了免疫系统的主要部分。植物先天免疫依赖于核苷酸结合的富亮氨酸重复受体（NLRs），其识别病原体来源的分子并激活下游信号通路。我们分析了NLR基因拷贝数的变异，并鉴定了相对于姐妹种NLR基因数量较少的植物。我们特别关注了来自两个不同谱系的四种植物，一个单子叶谱系（泽泻属）和一个双子叶谱系（扁桃科）。在这些谱系中，NLR基因的缺失与众所周知的下游免疫信号复合物ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)/PHYTOALEXIN DEFICIENT 4 （PAD4）的缺失同时发生。我们将分析扩展到整个蛋白质组，发现其他特征免疫基因仅在扁桃科和泽泻科中缺失。此外，我们在5个植物物种中发现了与EDS1/PAD4一起收敛丢失的未知功能基因。对拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）的基因表达分析表明，候选植物的几个同源物在病原体感染、干旱和脱落酸处理时存在差异表达。我们的分析为某些植物谱系中植物免疫的重新连接，以及EDS1/PAD4通路与干旱反应的共同进化提供了进化证据。
Genome analyses reveal that several plants have lost a major part of the immune system. Plant innate immunity relies on nucleotide binding leucine-rich repeat receptors (NLRs) that recognize pathogen-derived molecules and activate downstream signaling pathways. We analyzed the variation in NLR gene copy number and identified plants with a low number of NLR genes relative to sister species. We specifically focused on four plants from two distinct lineages, one monocot lineage (Alismatales) and one eudicot lineage (Lentibulariaceae). In these lineages, the loss of NLR genes coincides with loss of the well-known downstream immune signaling complex ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)/PHYTOALEXIN DEFICIENT 4 (PAD4). We expanded our analysis across whole proteomes and found that other characterized immune genes were absent only in Lentibulariaceae and Alismatales. Additionally, we identified genes of unknown function that were convergently lost together with EDS1/PAD4 in five plant species. Gene expression analyses in Arabidopsis (Arabidopsis thaliana) and Oryza sativa revealed that several homologs of the candidates are differentially expressed during pathogen infection, drought, and abscisic acid treatment. Our analysis provides evolutionary evidence for the rewiring of plant immunity in some plant lineages, as well as the coevolution of the EDS1/PAD4 pathway and drought responses.