A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam
A new entomopathogenic nematode, Steinernema robustispiculum n. sp. (Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam
复制标题
一种新的昆虫病原线虫，斯氏线虫（SteinernemaRobustispiculum n.）。
DOI：
10.1007/s11230-004-1373-x
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发表时间：
2004
影响因子：
1.3
通讯作者：
M. Moens
中科院分区：
文献类型：
--
作者：
L. K. Phan;S. Subbotin;L. Waeyenberge;M. Moens
Steinernemar robustispiculumn. sp. (Rhabditida: Steinernematidae) was isolated from woodland in Chumomray National Park, Sason, Sathay, Kontum, Vietnam. Its morphology, morphometrics, cross-hybridisation and the ITS-rDNA sequence analysis revealed thatS. robustispiculumclearly differs from other knownSteinernemaspp. As in the cases ofS. intermedium(Poinar, 1985),S. robustispiculumhas very robust spicules, but it can be distinguished by the longer tail of the infective juvenile, lower E%, shorter spicules, the shape of the spicules, the number of genital papillae in the caudal region and the presence of a mucron on the male tail.S. robustispiculumhas a lateral field resembling that ofS. sangiPhan, Nguyen & Moens, 2001, but can be distinguished by a higher E%, higher D%, smaller length to width ratio of the spicules and the morphology of both the spicule head (manubrium) and the dorsal lobe of the spicule. The morphometrics of infective juveniles ofS. robustispiculumare similar to those ofS. monticolumStock, Choo & Kaya, 1997; these species can be distingusihed by the position of the excretory pore, the smaller length to width ratio of the spicules, and the length and morphology of the spicule head (manubrium). The phylogenetic relationships withinSteinernemaTravassos, 1927, including the newly sequenced Vietnamese speciesS. robustispiculumn. sp.,S. lociPhan, Nguyen & Moens, 2001,S. thanhiPhan, Nguyen & Moens, 2001 andS. sangi, are presented based on analyses of the ITS-rDNA. The ITS RFLP profiles obtained from 17 different restriction enzymes are also presented.
