The nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (EC 2.7.7.6) of Agrobacterium tumefaciens (Smith and Townsend) Conn. Purification and properties of the enzyme from the tumorigenic strain B6806.
The nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (EC 2.7.7.6) of Agrobacterium tumefaciens (Smith and Townsend) Conn. Purification and properties of the enzyme from the tumorigenic strain B6806.
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根癌农杆菌（Smith 和 Townsend）康涅狄格州的核苷三磷酸核糖核酸核苷酸转移酶 (EC 2.7.7.6)。来自致瘤菌株 B6806 的酶的纯化和特性。
DOI：
10.1042/bj1430511
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发表时间：
1974
影响因子：
4.1
通讯作者：
U. C. Knopf
中科院分区：
文献类型：
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作者：
U. C. Knopf
纯化了植物致瘤细菌根癌农杆菌的RNA核苷酸转移酶（RNA聚合酶）。该方法包括在Waring Blendor中用玻璃珠破碎细菌细胞，用DEAE-纤维素处理，用（NH（4））（2）SO（4）分级分离，硫酸鱼精蛋白沉淀，DEAE-纤维素柱层析和甘油梯度离心或磷酸纤维素层析。高度纯化的酶的亚基结构与大肠杆菌的RNA核苷酸转移酶相似，但不相同。它可以被描述为beta '、beta'、chi（1）和alpha（mol.wts.分别为160000、150000、98000和41000+/-10%）。chi（1）是蛋白质亚基的临时名称，其可能与E中的sigma亚基具有相同的功能。杆菌A.根癌农杆菌对利福平敏感，体外最适温度为41+/-1 ℃，最适pH为8.2+/-0.1。Mg（2+）和Mn（2+）是活化剂。该酶以不同的效率转录人工、病毒、细菌、植物和动物模板。
The RNA nucleotidyltransferase (RNA polymerase) of the plant-tumorigenic bacterium Agrobacterium tumefaciens was purified. The method involves the disruption of the bacterial cells with glass beads in a Waring Blendor, treatment with DEAE-cellulose, fractionation with (NH(4))(2)SO(4), protamine sulphate precipitation, DEAE-cellulose column chromatography and either glycerol-gradient centrifugation or phosphocellulose chromatography. The subunit structure of the highly purified enzyme is similar to, although not identical with, the RNA nucleotidyltransferase of Escherichia coli. It can be described as beta', beta, chi(1) and alpha (mol.wts. 160000, 150000, 98000, and 41000+/-10% respectively). chi(1) is the temporary designation for a protein subunit, which might have the same functions as the sigma subunit in E. coli. The enzyme of A. tumefaciens is rifampicin-sensitive, has a temperature optimum in vitro of 41+/-1 degrees C and a pH optimum of 8.2+/-0.1. Mg(2+) and Mn(2+) are activators. The enzyme transcribes with different efficiencies artificial, viral, bacterial, plant and animal templates.