Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth
Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth
复制标题
水稻纤维素合酶样 D4 对于正常细胞壁生物合成和植物生长至关重要
DOI：
10.1111/j.1365-313x.2009.04022.x
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发表时间：
2009-12-01
期刊：
影响因子：
7.2
通讯作者：
Zhou, Yihua
中科院分区：
文献类型：
--
作者：
Li, Ming;Xiong, Guangyan;Zhou, Yihua
糖基转移酶家族2（GT 2）的纤维素转移酶样（CSL）蛋白被认为参与细胞壁聚合物的生物合成。CSL D亚家族（CSLD）是所有植物共有的，但CSLDs的功能仍有待阐明。我们在这里报告了一个窄叶和矮秆1（nd 1）水稻突变体，表现出显着减少植物生长，由于细胞分裂迟缓的深入表征。图位克隆结果表明，ND 1编码水稻CSLD亚家族的OsCSLD 4。OsCSLD 4主要在快速生长的组织中表达。在水稻原生质体细胞或烟草叶片中表达的C-或N-末端荧光标记的OsCSLD 4蛋白表明，该蛋白位于内质网或高尔基体囊泡中。使用在其自身启动子控制下表达OsCSLD 4-GUS的表型拯救转基因植物验证高尔基体定位。两个表型改变的组织，秆和根尖，被用来调查特定的壁缺陷。免疫学研究和单糖组成和糖基连接分析探索了由OsCSLD 4破坏引起的几种壁组成效应，包括阿拉伯木聚糖结构的改变以及纤维素和同型半乳糖醛酸的含量，这在单子叶草物种水稻（水稻）中是不同的。这两种组织中不一致的改变和初生壁中可观察到的结构缺陷表明OsCSLD 4在细胞壁形成和植物生长中起重要作用。
P>Cellulose synthase-like (CSL) proteins of glycosyltransferase family 2 (GT2) are believed to be involved in the biosynthesis of cell-wall polymers. The CSL D sub-family (CSLD) is common to all plants, but the functions of CSLDs remain to be elucidated. We report here an in-depth characterization of a narrow leaf and dwarf1 (nd1) rice mutant that shows significant reduction in plant growth due to retarded cell division. Map-based cloning revealed that ND1 encodes OsCSLD4, one of five members of the CSLD sub-family in rice. OsCSLD4 is mainly expressed in tissues undergoing rapid growth. Expression of OsCSLD4 fluorescently tagged at the C- or N-terminus in rice protoplast cells or Nicotiana benthamiana leaves showed that the protein is located in the endoplasmic reticulum or Golgi vesicles. Golgi localization was verified using phenotype-rescued transgenic plants expressing OsCSLD4-GUS under the control of its own promoter. Two phenotype-altered tissues, culms and root tips, were used to investigate the specific wall defects. Immunological studies and monosaccharide compositional and glycosyl linkage analyses explored several wall compositional effects caused by disruption of OsCSLD4, including alterations in the structure of arabinoxylan and the content of cellulose and homogalacturonan, which are distinct in the monocot grass species Oryza sativa (rice). The inconsistent alterations in the two tissues and the observable structural defects in primary walls indicate that OsCSLD4 plays important roles in cell-wall formation and plant growth.