Molecular and Phenotypic Characterization Revealed High Prevalence of Multidrug-Resistant Methicillin-Susceptible Staphylococcus aureus in Chongqing, Southwestern China
Molecular and Phenotypic Characterization Revealed High Prevalence of Multidrug-Resistant Methicillin-Susceptible Staphylococcus aureus in Chongqing, Southwestern China
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分子和表型特征揭示中国西南部重庆地区多重耐药甲氧西林敏感金黄色葡萄球菌的高流行率
DOI：
10.1089/mdr.2016.0078
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发表时间：
2017-03-01
影响因子：
2.6
通讯作者：
Rao, Xiancai
中科院分区：
文献类型：
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作者：
Yang, Yancheng;Hu, Zhen;Rao, Xiancai
甲氧西林敏感金黄色葡萄球菌（MSSA）约占中国葡萄球菌感染的40%。然而，目前对MSSA的分子特性还没有很好的描述。本研究对2013年在重庆市某综合性教学医院采集的124株MSSA进行了抗生素药敏试验和分子分型，包括多位点序列分型、葡萄球菌蛋白a （spa）基因分型、辅助基因调节因子（agr）分型、脉冲场凝胶电泳（PFGE）分型、潘通-瓦朗丁白细胞介素（pvl）基因检测、耐药基因检测等。MSSA菌株具有较高的遗传异质性。共鉴定出10个PFGE组，26个序列类型，47个spa类型。I型（62.9%）是最常见的agr类型，其次是II型（15.3%）、IV型（11.3%）和III型（10.5%）。pvl基因阳性率为27.4%（34/124）。值得注意的是，44.4%（55/124）的MSSA菌株存在多药耐药（MDR），其中MDR菌株主要对青霉素、红霉素和克林霉素耐药。84.7%的菌株存在抗性基因blaZ， 85.5%的菌株存在ermC， 28.2%的菌株存在ermA， 16.1%的菌株存在tetK， 6.5%的菌株存在tetM， 2.6%的菌株存在aacA-aphD。这些数据表明重庆地区MDR MSSA患病率较高，因此需要控制MSSA感染。
Methicillin-susceptible Staphylococcus aureus (MSSA) accounts for approximate to 40% of staphylococcal infections in China. However, the molecular characterization of MSSA is not well described. In this study, 124 MSSA strains collected in 2013 from a comprehensive teaching hospital in Chongqing, Southwestern China, were subjected to antibiotics susceptibility testing and molecular typing, including multilocus sequence typing, staphylococcal protein A (spa) gene typing, accessory gene regulator (agr) typing, pulsed-field gel electrophoresis (PFGE) typing, Panton-Valentine leukocidin (pvl) gene detection, and antibiotic-resistant gene detection. MSSA strains exhibited high genetic heterogeneity. A total of 10 PFGE groups, 26 sequence types, and 47 spa types were identified. Type I (62.9%) was the most frequent agr type, followed by type II (15.3%), type IV (11.3%), and type III (10.5%). The prevalence of pvl genes was 27.4% (34/124). Notably, 44.4% (55/124) of MSSA strains were multidrug resistance (MDR), and MDR isolates were mostly resistant to penicillin, erythromycin, and clindamycin. The resistance gene blaZ was present in 84.7% of strains, ermC was present in 85.5% of strains, ermA was present in 28.2% of strains, tetK was present in 16.1% of strains, tetM was present in 6.5% of strains, and aacA-aphD was present in 2.6% of strains. These data demonstrated the high prevalence of MDR MSSA in Chongqing, thereby indicating the need to control MSSA infection.