Post-Irradiated Human Submandibular Glands Display High Collagen Deposition, Disorganized Cell Junctions, and an Increased Number of Adipocytes
Post-Irradiated Human Submandibular Glands Display High Collagen Deposition, Disorganized Cell Junctions, and an Increased Number of Adipocytes
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DOI：
10.1369/0022155416646089
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发表时间：
2016-06-01
影响因子：
3.2
通讯作者：
Baker, Olga J.
中科院分区：
文献类型：
--
作者：
Nam, Kihoon;Maruyama, Christina L.;Baker, Olga J.
唾液腺对维持口腔健康至关重要。头颈部放射治疗是唾液腺功能减退最常见的原因之一。人们对放射治疗后唾液腺的结构变化知之甚少。这项研究的目的是了解照射后的人(颌下腺[SMG])与未经治疗的人相比发生的结构变化。我们测定了上皮极性的变化，胶原沉积的存在，以及脂肪组织的改变。我们使用了福尔马林固定的石蜡包埋人SMG，来自两名暴露于头部和颈部照射的女性受试者。苏木精-伊红染色和Masson三色染色。免疫组织切片用共聚焦显微镜观察。用ImageJ、Prism和SPSS软件计算每张组织切片中脂肪细胞的数量和大小。与未照射的对照组相比，照射后的人SMG显示高胶原沉积，细胞连接紊乱，脂肪细胞数量增加。这些发现对于提高我们对与辐射相关的唾液腺功能障碍的个体风险和变异的理解是重要的。
Salivary glands are vital for maintaining oral health. Head and neck radiation therapy is one of the most common causes of salivary gland hypofunction. Little is known about the structural changes that occur in salivary glands after radiation therapy. The aim of this study is to understand the structural changes that occur in post-irradiated human (submandibular gland [SMG]) as compared with untreated ones. We determined changes in epithelial polarity, presence of collagen deposition, and alteration in adipose tissue. We used formalin-fixed paraffin-embedded human SMG from two female subjects exposed to head and neck irradiation. We utilized hematoxylin and eosin staining and Masson's Trichrome staining. The immunostained tissue sections were examined using confocal microscopy. The number and size of adipocytes per tissue section were calculated using ImageJ, Prism, and SPSS software. Post-irradiated human SMG displayed high collagen deposition, disorganized cell junctions, and an increased number of adipocytes as compared with non-irradiated controls. These findings are important to improve our understanding of the individual risk and variation in radiation-related salivary gland dysfunction.