An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens
An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens
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DOI：
10.1016/j.mimet.2005.03.005
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发表时间：
2005-10-01
影响因子：
2.2
通讯作者：
Forbes, KJ
中科院分区：
文献类型：
--
作者：
Thomson, LM;Traore, H;Forbes, KJ
A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.
