Let's push things forward: disruptive technologies and the mechanics of tissue assembly.
Let's push things forward: disruptive technologies and the mechanics of tissue assembly.
复制标题
让我们推动事情向前发展：颠覆性技术和组织组装机制。
DOI：
10.1039/c3ib40080h
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Nelson,CelesteM
中科院分区：
文献类型：
--
作者：
Varner,VictorD;Nelson,CelesteM
尽管已经描述了许多调节胚胎组织组装的分子机制，但将这些机制与组织形式的实际变化相结合的物理力仍然不清楚。定性研究表明，机械负荷在发育中起着调节作用，但缺乏明确的定量证据。这部分是由于这些问题的复杂性质-胚胎组织通常经历大的变形，并表现出不断变化的，高度粘弹性的材料特性。尽管存在这些挑战，但新的颠覆性技术正在以前所未有的细节研究组织组装的机制。在这里，我们提出了新的实验技术，使这些物理问题的每个组成部分的研究：运动学，力和本构特性。具体来说，我们详细介绍了光片显微镜，光学相干断层扫描，牵引力显微镜，荧光力光谱，微观流变学和微图案化的进展。总之，这些技术有助于阐明组织组装力学的更定量的理解。
Although many of the molecular mechanisms that regulate tissue assembly in the embryo have been delineated, the physical forces that couple these mechanisms to actual changes in tissue form remain unclear. Qualitative studies suggest that mechanical loads play a regulatory role in development, but clear quantitative evidence has been lacking. This is partly owing to the complex nature of these problems – embryonic tissues typically undergo large deformations and exhibit evolving, highly viscoelastic material properties. Still, despite these challenges, new disruptive technologies are enabling study of the mechanics of tissue assembly in unprecedented detail. Here, we present novel experimental techniques that enable the study of each component of these physical problems: kinematics, forces, and constitutive properties. Specifically, we detail advances in light sheet microscopy, optical coherence tomography, traction force microscopy, fluorescence force spectroscopy, microrheology and micropatterning. Taken together, these technologies are helping elucidate a more quantitative understanding of the mechanics of tissue assembly.