Gypsy-like retrotransposons are widespread in the plant kingdom
Gypsy-like retrotransposons are widespread in the plant kingdom
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DOI：
10.1046/j.1365-313x.1998.00071.x
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发表时间：
1998-03-01
期刊：
影响因子：
7.2
通讯作者：
Schulman, AH
中科院分区：
文献类型：
--
作者：
Suoniemi, A;Tanskanen, J;Schulman, AH
逆转录转座子通过RNA中间体传播，然后RNA中间体被逆转录并包装成病毒样颗粒。在编码结构域的顺序和序列相似性上，它们要么是复制式的，要么是吉普赛式的，吉普赛式的元件与逆转录病毒共享它们的组织结构，但缺乏逆转录病毒包膜结构域。复制样逆转录转座子，或者至少是它们的逆转录酶结构域，在高等植物中广泛分布，但吉普赛样元件仅在分散的物种中被报道。作者利用这些群体之间结构域顺序的差异来扩增和克隆连接特异吉普赛样逆转录转座子的逆转录酶-整合酶区域的片段。代表高等植物多样性的物种产生的产物序列表明，吉普赛样转座子分布在整个植物基因组中。这类植物元件被命名为罗曼尼反转录转座子。这两种类型在真菌、植物和动物中无处不在的存在，支持它们作为古老的独特谱系和随后的垂直辐射而存在。
Retrotransposons propagate via an RNA intermediate which is then reverse-transcribed and packaged into viruslike particles. They are either copia-or gypsy like in coding domain order and sequence similarity, the gypsy-like elements sharing their organization with the retroviruses but lacking retroviral envelope domains. Copia-like retrotransposons, or at least their reverse transcriptase domains, appear broadly distributed in higher plants, but gypsy-like elements have been reported only for scattered species. The authors have exploited the difference in domain order between these groups to amplify and clone segments bridging the reverse transcriptase - integrase region of specifically gypsy-like retrotransposons. Species representative of the diversity of higher plants yielded products whose sequences establish that gypsy like transposons are dispersed throughout the plant genomes. This class of plant elements has been named romani retrotransposons. The presence of both types ubiquitously in the fungi, plants and animals support their existence as ancient distinct lineages and subsequent, vertical radiation.