Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance
Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance
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DOI：
10.1016/j.bbrc.2003.11.119
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发表时间：
2004-01-09
影响因子：
3.1
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Urano, K;Yoshiba, Y;Shinozaki, K
精氨酸脱羧酶（ADC）催化多胺（PA）生物合成的第一步，即由精氨酸（Arg）生成腐胺（Put）。拟南芥的2个ADC基因之一，AtADC 2，响应于盐胁迫而被诱导，引起游离Put的积累。为了分析胁迫诱导型AtADC 2基因和内源Put在植物耐盐性中的作用，我们分离了一个AtADC 2基因Ds插入突变体（adc 2 -1），并对其在盐胁迫下的表型进行了分析。在adc 2 -1突变体中，游离Put含量降低到对照植物中的约25%，并且在盐胁迫下没有增加。此外，adc 2 -1突变体对盐胁迫的敏感性高于对照。外源Put的加入可恢复adc 2 -1的胁迫敏感性。这些结果表明，内源Put在拟南芥的耐盐性中起着重要作用。AtADC 2基因不仅在盐胁迫条件下，而且在正常条件下都是Put产生的关键基因。(C)2003年爱思唯尔公司All rights reserved.
Arginine decarboxylase (ADC) catalyzes the first step of polyamine (PA) biosynthesis to produce putrescine (Put) from arginine (Arg). One of the 2 Arabidopsis ADC genes, AtADC2, is induced in response to salt stress causing the accumulation of free Put. To analyze the roles of stress-inducible AtADC2 gene and endogenous Put in stress tolerance, we isolated a Ds insertion mutant of AtADC2 gene (adc2-1) and characterized its phenotypes under salt stress. In the adc2-1 mutant, free Put content was reduced to about 25% of that in the control plants and did not increase under salt stress. Furthermore, the adc2-1 mutant was more sensitive to salt stress than the control plants. The stress sensitivity of adc2-1 was recovered by the addition of exogenous Put. These results indicate that endogenous Put plays an important role in salt tolerance in Arabidopsis. AtADC2 is a key gene for the production of Put under not only salinity conditions, but also normal conditions. (C) 2003 Elsevier Inc. All rights reserved.