Surface modification of silica nanoparticles to reduce aggregation and nonspecific binding
Surface modification of silica nanoparticles to reduce aggregation and nonspecific binding
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DOI：
10.1021/la052797j
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发表时间：
2006-04-25
期刊：
影响因子：
3.9
通讯作者：
Tan, WH
中科院分区：
文献类型：
--
作者：
Bagwe, RP;Hilliard, LR;Tan, WH
本文对二氧化硅纳米颗粒表面修饰方案的设计和开发进行了系统的研究。纳米颗粒表面设计涉及使用惰性和活性表面官能团的最佳平衡，以实现最小的纳米颗粒聚集并减少纳米颗粒的非特异性结合。在油包水微乳液中制备了二氧化硅纳米粒子，并用正硅酸乙酯(TEOS)和各种有机硅烷试剂对其进行了表面改性。制备了具有不同官能团的纳米粒子，包括羧酸盐、胺、胺/膦酸盐、聚乙二醇、十八基和羧酸盐/十八基。利用扫描电子显微镜、动态光散射和Zeta电位分析进行的聚集研究表明，通过在表面添加惰性官能团，如甲基膦酸甲酯，可以减少胺修饰的二氧化硅纳米颗粒之间的严重聚集。为了确定不同的表面修饰方案对纳米颗粒非特异性结合的影响，还利用共聚焦成像/荧光显微镜研究了功能化的二氧化硅纳米颗粒与DNA芯片之间的相互作用。带有十八基和羧酸基的染料掺杂二氧化硅纳米颗粒表现出最小的非特异性结合。使用这些表面修饰方案，荧光染料掺杂的二氧化硅纳米颗粒可以更容易地与生物分子结合，并在生物分析应用中用作高荧光、高灵敏度和可重复性的标记。
In this article, a systematic study of the design and development of surface-modification schemes for silica nanoparticles is presented. The nanoparticle surface design involves an optimum balance of the use of inert and active surface functional groups to achieve minimal nanoparticle aggregation and reduce nanoparticle nonspecific binding. Silica nanoparticles were prepared in a water-in-oil microemulsion and subsequently surface modified via cohydrolysis with tetraethyl orthosilicate (TEOS) and various organosilane reagents. Nanoparticles with different functional groups, including carboxylate, amine, amine/phosphonate, poly(ethylene glycol), octadecyl, and carboxylate/octadecyl groups, were produced. Aggregation studies using SEM, dynamic light scattering, and zeta potential analysis indicate that severe aggregation among amine-modified silica nanoparticles can be reduced by adding inert functional groups, such as methyl phosphonate, to the surface. To determine the effect of various surface-modification schemes on nanoparticle nonspecific: binding, the interaction between functionalized silica nanoparticles and a DNA chip was also studied using confocal imaging/fluorescence microscopy. Dye-doped silica nanoparticles functionalized with octadecyl and carboxylate groups showed minimal nonspecific binding. Using these surface-modification schemes, fluorescent dye-doped silica nanoparticles can be more readily conjugated with biomolecules and used as highly fluorescent, sensitive, and reproducible labels in bioanalytical applications.