The circadian rhythm of expression of Bmal1 and Cry1 in peripheral blood mononuclear cells of preterm neonates
The circadian rhythm of expression of Bmal1 and Cry1 in peripheral blood mononuclear cells of preterm neonates
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DOI：
10.3109/14767051003677947
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发表时间：
2010-10-01
影响因子：
1.8
通讯作者：
Chen, An
中科院分区：
文献类型：
--
作者：
Chen, An
Objective.探讨早产儿外周血单个核细胞中昼夜节律基因的昼夜节律。10例早产儿，胎龄30 ~ 31周。排除有出生窒息、RDS、呼吸暂停、畸形、感染或溶血性疾病的婴儿。分别于入院第1、7、14天上午7点、下午7点抽取早产儿外周静脉血，采用实时荧光定量PCR法检测Bmal 1、Cry 1的表达。第1、7、14天上午7点与下午7点bmal 1表达差异无统计学意义。也没有哭。结论。新生儿重症监护病房早产儿PBMC中bmal 1和cry 1的表达无昼夜节律。
Objective. To determine circadian rhythms of circadian genes in the peripheral blood mononuclear cells in preterm neonates.Study design. Ten premature neonates, gestational age from 30 to 31 weeks were recruited. Infants with birth asphyxia, RDS, apnea, malformation, infection or haemolytic diseases were excluded. At 7AM and 7PM on the 1st day, 7th day and 14th day of hospitalisation, peripheral venous blood was obtained from the premature babies and real-time RTPCR was used to determine the expression of Bmal1 and Cry1.Results. The expression of bmal1 did not have statistical difference between 7AM and 7PM on the 1st day, 7th day and 14th day. Neither did the cry1.Conclusion. There was no circadian rhythm of bmal1 and cry1 in PBMC of preterm neonates in our NICU.