Comparison of Lysophospholipid Levels in Rat Feces with Those in a Standard Chow
Comparison of Lysophospholipid Levels in Rat Feces with Those in a Standard Chow
复制标题
DOI：
10.1021/jf200986k
复制
发表时间：
2011-07-13
影响因子：
6.1
通讯作者：
Tokumura, Akira
中科院分区：
文献类型：
--
作者：
Inoue, Manami;Adachi, Mika;Tokumura, Akira
尽管溶血磷脂通过其特定的受体具有不同的生理活性，引起了人们的广泛关注，但人们对其作为一种次要食物成分在哺乳动物消化系统中的代谢命运知之甚少。在本研究中，我们用二维薄层层析和液-质联用法分析了标准大鼠饲料的脂肪提取物和饲喂该饲料的大鼠粪便中的5种溶血磷脂。大鼠饲料中溶血磷脂含量最高的是溶血磷脂酰胆碱，其次是溶血磷脂酰乙醇胺、溶血磷脂酸(LPA)、溶血磷脂酰肌醇和溶血磷脂酰丝氨酸(LPS)，但在大鼠粪便中含量很低。在食物中的内毒素分子种类中，只有在粪便中检测到大量的饱和种类。此外，几种LPA分子物种以不同的比例留在粪便中(饱和>；单不饱和>；多不饱和)。这些结果表明，摄入的LPA和内毒素的一部分到达了大鼠的大肠，影响了生理结肠功能。
Although lysophospholipids have attracted much attention due to their diverse physiological activities through their specific receptors, little is known about their metabolic fates in mammalian digestive systems after their ingestion as a minor food component. In this study, we analyzed five lysophospholipids in lipid extracts of a standard rat chow and feces of rats fed the chow by two-dimensional thin layer chromatography and liquid chromatography tandem mass spectrometry. The most abundant lysophospholipid in the rat chow was lysophosphatidylcholine followed by lysophosphatidylethanolamine, lysophosphatidic acid (LPA), lysophosphatidylinositol and lysophosphatidylserine (LPS) in an increasing order, but their concentrations were very low in rat feces. Among the molecular species of LPS in the chow, only saturated species were detected in the feces in significant amounts. In addition, several molecular species of LPA remained in the feces in variable portions (saturated > monounsaturated > polyunsaturated). These results suggest that a portion of ingested LPA and LPS reach the rat large intestine, affecting physiological colon functions.