Expression and Subcellular Localization of Metastasis-Associated Protein 1, Its Short Form, and Estrogen Receptors in Rat Placenta
Expression and Subcellular Localization of Metastasis-Associated Protein 1, Its Short Form, and Estrogen Receptors in Rat Placenta
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DOI：
10.1177/1933719114549851
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发表时间：
2015-04-01
影响因子：
2.9
通讯作者：
Al-Abdallah, Abeer Adnan
中科院分区：
文献类型：
--
作者：
Al-Bader, Maie Dawoud;Kilarkaje, Narayana;Al-Abdallah, Abeer Adnan
转移相关蛋白1（MTA 1）及其短型（MTA 1 s）调节雌激素受体（ER）的功能。雌激素通过ER影响胎盘生长和胎儿发育，这一过程可能涉及MTA 1信号传导。在妊娠第16、19和21天（dg）研究了大鼠胎盘中MTA 1、MTA 1 s、ER和ER基因和蛋白的表达。雌激素受体信使RNA在妊娠末期显著下降，而其蛋白水平在21日龄时在核组分中增加。MTA 1和MTA 1 s均随妊娠增加而增加。所有dg的蜕膜、滋养层巨细胞、糖原和绒毛滋养层细胞均表达MTA 1、ER和ER蛋白，MTA 1与ER和ER共定位于细胞核和细胞质中。MTA 1的表达表明可能在调节胎盘功能中起作用;考虑到MTA 1对ER的抑制功能，MTA 1的表达表明妊娠晚期胎盘细胞对雌激素的敏感性可能较低。
Metastasis-associated protein 1 (MTA1) and its short form (MTA1s) regulate the function of estrogen receptors (ERs). Estrogens, via ERs, affect placental growth and fetal development, a process that may involve MTA1 signaling. Expression of MTA1, MTA1s, ER, and ER genes and proteins in rat placentas was studied on 16, 19, and 21 days of gestation (dg). The ER messenger RNA decreased significantly toward the end of gestation, whereas its protein level increased in the nuclear fraction on 21 dg. Both MTA1 and MTA1s increased with gestation. Decidual, trophoblast giant, glycogen, and villous trophoblast cells expressed MTA1, ER, and ER proteins on all dg with colocalization of MTA1 with ER and ER in the nucleus and cytoplasm. Expression of MTA1 suggests a possible role in regulating placental functions; considering the repressive function of MTA1 on ERs, the expression of MTA1 suggests that placental cells may be less sensitive to estrogens during late pregnancy.