Thermally Gated Bio-orthogonal Nanozymes with Supramolecularly Confined Porphyrin Catalysts for Antimicrobial Uses
Thermally Gated Bio-orthogonal Nanozymes with Supramolecularly Confined Porphyrin Catalysts for Antimicrobial Uses
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DOI：
10.1016/j.chempr.2020.01.015
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发表时间：
2020-05-14
期刊：
影响因子：
23.5
通讯作者：
Rotello, Vincent M.
中科院分区：
文献类型：
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作者：
Cao-Milan, Roberto;Gopalakrishnan, Sanjana;Rotello, Vincent M.
生物正交催化具有在体外和体内局部生成成像和治疗分子的能力。将这些催化剂整合到温敏纳米颗粒平台中将产生可通过内源性或外源性热控制来控制的生物正交“纳米酶”。我们通过将卟啉超分子组装体限制在单层金纳米颗粒中来制备温度响应性纳米酶。所得的纳米器件具有在3摄氏度范围内发生的开关门控热响应，具有从25摄氏度到37摄氏度的激活温度的相称可调性。在复杂的生物环境中证明了催化的可逆活化，并且通过基于抗生素的前药的热活化来有效地处理细菌生物膜，证明了双稳态温敏纳米酶的功效。
Bio-orthogonal catalysis has the capability of localized generation of imaging and therapeutic molecules in vitro and in vivo. The integration of these catalysts into thermoresponsive nanoparticle platforms would generate bio-orthogonal "nanozymes" that could be controlled through endogenous or exogenous thermal control. We have fabricated thermoresponsive nanozymes by confining supramolecular assemblies of porphyrins into the monolayer of gold nanoparticles. The resulting nanodevices feature an on-off gated thermal response occurring over a 3 degrees C range with commensurate tunability of activation temperature from 25 degrees C to 37 degrees C. Reversible activation of catalysis was demonstrated in complex biological environments, and the efficacy of bi-stable thermoresponsive nanozymes demonstrated through thermal activation of antibiotic-based prodrugs to effectively treat bacterial biofilms.