MODIFICATION OF LYMPHOCYTE MIGRATION BY SULFATED POLYSACCHARIDES
MODIFICATION OF LYMPHOCYTE MIGRATION BY SULFATED POLYSACCHARIDES
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DOI：
10.1002/eji.1830160419
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发表时间：
1986-04-01
影响因子：
5.4
通讯作者：
PARISH, CR
中科院分区：
文献类型：
--
作者：
BRENAN, M;PARISH, CR
硫酸多糖在淋巴细胞迁移中的作用已经在体内使用细胞内DNA结合荧光染料Hoechst 33342标记的淋巴细胞进行了分析。一组硫酸化多糖对进入（通过在标记细胞之前注射硫酸化多糖）和从淋巴器官移位（通过在标记细胞定位后注射硫酸化多糖）的影响表明，不同的硫酸化多糖对进入和移位以及器官内亚群的定位具有选择性作用。另外的实验表明，高内皮微静脉上的硫酸多糖受体可能与淋巴细胞上的互补结构相互作用。支持这一结论的数据是：（a）与硫酸多糖预孵育的淋巴细胞的正常定位行为;（B）硫酸多糖的淋巴细胞表面受体的表达与淋巴细胞进入淋巴器官的能力之间的反比关系;（c）硫酸多糖偶联的荧光素化珠与高内皮微静脉的选择性结合。在这种情况下，只有与抑制进入的硫酸多糖偶联的珠与高内皮微静脉结合。这些研究结果进行了讨论的一个基本的细胞识别系统，利用硫酸多糖。
The role of sulfated polysaccharides in lymphocyte migration has been analyzed in vivo using lymphocytes labeled with an intracellular DNA-binding fluorochrome Hoechst 33342. The influence of a panel of sulfated polysaccharides on entry (by injecting the sulfated polysaccharide prior to the labeled cells) and displacement from lymphoid organs (by injecting the sulfated polysaccharide after the labeled cells have localized) indicated that different sulfated polysaccharides have selective effects on entry and displacement, and furthermore positioning of subpopulations within organs. Additional experiments suggested that receptors for sulfated polysaccharides on high endothelial venules may interact with complementary structures on lymphocytes. The data supporting this conclusion were: (a) the normal localization behavior of lymphocytes preincubated with sulfated polysaccharides; (b) an inverse relationship between the expression of lymphocyte surface receptors for sulfated polysaccharides and the ability of the lymphocytes to enter lymphoid organs and (c) the selective binding of sulfated polysaccharide-coupled fluoresceinated beads to high endothelial venules. In this case only the beads coupled with the sulfated polysaccharides that inhibited entry bound to the high endothelial venules. These findings are discussed in terms of a fundamental cellular recognition system utilizing sulfated polysaccharides.