Atelocollagen Sponge as a Stem Cell Implantation Scaffold for the Treatment of Scarred Vocal Folds
Atelocollagen Sponge as a Stem Cell Implantation Scaffold for the Treatment of Scarred Vocal Folds
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去端肽胶原海绵作为干细胞植入支架治疗疤痕声带
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
J. Ito
中科院分区：
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作者：
S. Ohno;S. Hirano;I. Tateya;S. Kanemaru;H. Umeda;A. Suehiro;Yoshiharu Kitani;Yo Kishimoto;Tsuyoshi Kojima;Tatsuo Nakamura;J. Ito
目的声带瘢痕的治疗仍然是一个治疗挑战。本课题组先前报道了通过植入含有间充质干细胞的骨髓基质细胞来治疗声带损伤的效果。合适的支架是干细胞移植达到最佳效果所必需的。Terudermis是一种从小牛真皮中提取的胶原蛋白海绵。它有大的毛孔，可以进入细胞，在体内被降解。这些特点表明，这种材料可能是一种很好的候选材料，用于细胞植入的支架。本体外研究探讨了Terudermis作为支架材料的可行性。方法从绿色荧光蛋白(GFP)小鼠股骨分离培养骨髓基质细胞。将细胞接种于Terudermis中，培养5d。观察细胞存活、增殖及细胞外基质表达情况。结果骨髓基质细胞与Terudermis黏附，并有明显的增殖。免疫组织化学染色显示贴壁细胞呈波形蛋白、结蛋白、纤维连接蛋白和fsp1阳性表达，βIII微管蛋白表达阴性。这些发现表明，这些细胞是中胚层细胞，并在生物学上附着于atelocollage纤维。结论Terudermis有可能作为干细胞植入支架用于治疗声带瘢痕。
Objectives Treatment of vocal fold scarring remains a therapeutic challenge. Our group previously reported the efficacy of treating injured vocal folds by implantation of bone marrow–derived stromal cells containing mesenchymal stem cells. Appropriate scaffolding is necessary for the stem cell implant to achieve optimal results. Terudermis is an atelocollagen sponge derived from calf dermis. It has large pores that permit cellular entry and is degraded in vivo. These characteristics suggest that this material may be a good candidate for use as scaffolding for implantation of cells. The present in vitro study investigated the feasibility of using Terudermis as such a scaffold. Methods Bone marrow–derived stromal cells were obtained from GFP (green fluorescent protein) mouse femurs. The cells were seeded into Terudermis and incubated for 5 days. Their survival, proliferation, and expression of extracellular matrix were examined. Results Bone marrow–derived stromal cells adhered to Terudermis and underwent significant proliferation. Immunohistochemical examination demonstrated that adherent cells were positive for expression of vimentin, desmin, fibronectin, and fsp1 and negative for beta III tubulin. These findings indicate that these cells were mesodermal cells and attached to the atelocollagen fibers biologically. Conclusions The data suggest that Terudermis may have potential as stem cell implantation scaffolding for the treatment of scarred vocal folds.