Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 1. Synthesis and characterization.
Tissue-culture surfaces with mixtures of aminated and fluorinated functional groups. Part 1. Synthesis and characterization.
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具有胺化和氟化官能团混合物的组织培养表面。
DOI：
10.1163/156856203321478856
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Hoffman,AllanS
中科院分区：
文献类型：
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作者：
Bain,JamesR;Hoffman,AllanS
培养皿的表面化学可以对培养细胞的表型产生深远的影响。在本研究中，有机硅烷从水中的二元混合物在硼硅酸盐玻璃上的化学吸附产生了含有二胺基团(N2)、三氟丙基(F3)以及这两种基团的混合物的表面。N_2-F_3表面的组成由硅烷化溶液中单体的比例控制，化学分析的电子光谱和表面胺与荧光素-5-异硫氰酸酯的偶联证实了这一点。原子力显微镜显示硅烷化表面是片状的，尽管它们的均方根粗糙度与光滑玻璃(0.3 nm)没有显著差异。二胺残基最丰富的表面是最亲水的，水接触角≤提前90°。随附的论文(本期的下一篇文章)描述了这些表面化学物质对体外转基因胰岛素瘤细胞表型的影响。我们的结论是，从N_2-F_3体系中的化学吸附为玻璃培养表面的化学裁剪提供了一种简单的、一次性的方法。
Surface chemistry of culture dishes can have profound effects on the phenotype of cultured cells. In the present study, chemisorption from aqueous, binary mixtures of organosilanes onto borosilicate glass created surfaces bearing diamine groups (N2), trifluoropropyl groups (F3) and mixtures of the two. Composition of N2-F3 surfaces was controlled by the ratio of monomers in the silanization bath, as confirmed by electron spectroscopy for chemical analysis and by conjugation of surface amines with fluorescein-5-isothiocyanate. Atomic-force microscopy revealed that silanized surfaces are patchy, though their root-mean-square roughnesses do not differ significantly from that of smooth glass (0.3 nm). Surfaces richest in diamine residues were the most hydrophilic, with advancing water-contact angles ≤90°. The accompanying paper (the next article in this issue) describes the effects of these surface chemistries on the phenotype of transgenic insulinoma cellsin vitro. We conclude that chemisorption from the N2-F3 system provides a simple, one-pot method for tailoring the chemistry of glass culture surfaces.