Molecular cytogenetic identification and relationship of the artificial intergeneric hybrid between Dendranthema indica and Crossostephium chinense by GISH
Molecular cytogenetic identification and relationship of the artificial intergeneric hybrid between Dendranthema indica and Crossostephium chinense by GISH
复制标题
DOI：
10.1007/s00606-010-0337-7
复制
发表时间：
2010-07
影响因子：
1.9
通讯作者：
F. Tang;Fadi Chen;Sumei Chen;Xiu-e Wang;Hongbo Zhao
中科院分区：
文献类型：
--
作者：
F. Tang;Fadi Chen;Sumei Chen;Xiu-e Wang;Hongbo Zhao
虽然大多数杂种胚在早期发育阶段败育，但一个2n= 27的真属间杂种Dendranthema indica（L.）Des Moul（2n= 36），为中国石楠（Crossostephium chinense（L.）Makino（2n= 18）是继代授粉和胚珠抢救后产生的一个新品种。由此产生的成年推定的混合动力车和两个父母的形态显着不同，从另一个在7个测量的9个性状，例外的叶宽和叶长，推定的混合动力车是无法区分的母本植物。基因组原位杂交实验能够成功地区分有丝分裂和减数分裂中期染色体的基因组起源的混合。此外，18号籼型染色体在减数分裂中期以二价体配对。
Although most of the hybrid embryos aborted at an early developmental stage, a 2n= 27 true intergeneric hybrid betweenDendranthema indica(L.) Des Moul (2n= 36) as ♀ andCrossostephium chinense(L.) Makino (2n= 18) as ♂ was produced following pollination and ovule rescue. The morphology of the resulting adult putative hybrid and the two parents differed significantly from one another in seven of the nine traits measured, the exceptions being leaf width and leaf length, for which the putative hybrid was indistinguishable from the maternal plant. Genomic in situ hybridization experiments were able to successfully distinguish the genomic origin of both mitotic and meiotic metaphase chromosomes in the hybrid. In addition, the 18D. indicachromosomes were paired as bivalents at meiotic metaphase in the hybrid.