An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement
An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement
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DOI：
10.1016/j.biomaterials.2003.10.078
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Schnettler, R
中科院分区：
文献类型：
--
作者：
Alt, V;Bechert, T;Schnettler, R
多重耐药菌感染已成为关节置换术中的一个严重问题。本研究报道了纳米银（NanoSilver）负载聚甲基丙烯酸甲酯骨水泥对多重耐药菌的体外抗菌活性和体外细胞毒性。epidermidis、耐甲氧西林S.表皮葡萄球菌（MRSE）和耐甲氧西林S.金黄色葡萄球菌（MRSA）的研究通过微孔板增殖试验。体外细胞毒性实验采用定量洗脱试验和定性生长试验，结果表明纳米银骨水泥对所有菌株（包括MRSE和MRSA）均表现出较高的抗菌活性。庆大霉素骨水泥对MRSA和MRSE无效，因为受试菌株的庆大霉素耐药性较高。普通水泥没有抑制任何菌株的增殖。纳米银的体外细胞毒性与无毒性对照无显著差异，载银盐骨水泥的细胞毒性使得这种银在过去不适合全天临床使用。这种称为纳米银的新型银在体外没有细胞毒性，并且对多重耐药细菌显示出高效性。如果结果可以在体内证实，纳米银可能在关节成形术中具有很高的兴趣。(C)2003爱思唯尔有限公司。保留所有权利。
Infections with multiresistant bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity against multiresistant bacteria and in vitro cytotoxicity of polymethylmetacrylate bone cement loaded with metallic silver particles with a size of 5-50 nm called NanoSilver.In vitro antibacterial activity against S. epidermidis, methicillin-resistant S. epidermidis (MRSE), and methicillin-resistant S. aureus (MRSA) was studied by microplate proliferation tests. Quantitative elution testing and qualitative ongrowth of human osteoblasts was done to study in vitro cytotoxicity.Only NanoSilver cement showed high-antibacterial activity against all strains, including MRSE and MRSA. Gentamicin cement was not effective against MRSA and MRSE due to the high-level gentamicin resistance of the tested strains. Plain cement did not inhibit proliferation of any strains. There was no significant difference regarding in vitro cytotoxicty between NanoSilver and the non-toxic control.Cytotoxicity of cement loaded with silver salts made this kind of silver unsuitable for all day clinical use in the past. This new form of silver called NanoSilver was free of in vitro cytotoxicity and showed high effectiveness against multiresistant bacteria. If the results can be confirmed in vivo NanoSilver may have a high interest in joint arthroplasty. (C) 2003 Elsevier Ltd. All rights reserved.