Mucosal antibodies to human cytomegalovirus glycoprotein B occur following both natural infection and immunization with human cytomegalovirus vaccines
Mucosal antibodies to human cytomegalovirus glycoprotein B occur following both natural infection and immunization with human cytomegalovirus vaccines
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DOI：
10.1093/infdis/174.2.387
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发表时间：
1996-08-01
影响因子：
6.4
通讯作者：
Plotkin, SA
中科院分区：
文献类型：
--
作者：
Wang, JB;Adler, SP;Plotkin, SA
由于抗人巨细胞病毒 (HCMV) 糖蛋白 B (gB) 的抗体会中和，因此在唾液和鼻腔冲洗液中测量了针对 HCMV 的 IgG、分泌型 IgA (sIgA) 和粘膜 IgA1 抗体的水平。 10 名血清阴性成人缺乏这些抗体，但在 10 名自然血清阳性成人中，10 名具有 Ige 至 gB，5 名具有 sIgA，0 名具有粘膜 IgA。在12名HCMV活疫苗接种者中，在免疫后10-20个月收集的样本中，8人有Ige至gB，4人有sIgA，2人有粘膜IgA；在 10 名 gB 疫苗接种者中，在加强接种前或加强接种后 1 个月收集的样本中，8 名接种者检测到 gB IgG，7 名接种者检测到 sIgA，7 名接种者检测到粘膜 IgA。血清中的 IgG 至 gB 和中和滴度与粘膜样本中的 IgG 至 gB 相关。 26 名受试者中有 25 名的唾液中含有 Ige 至 gB，其血清中和滴度 >1:64。血清中和滴度 >1:64，无论是由疫苗还是野生型病毒诱导，都与粘膜 Ige 与 HCMV 相关。
Because antibodies against human cytomegalovirus (HCMV) glycoprotein B (gB) neutralize, levels of IgG, secretory IgA (sIgA), and mucosal IgA1 antibodies to HCMV were measured in saliva and nasal washes. Ten seronegative adults lacked these antibodies, but of 10 naturally seropositive adults, 10 had Ige to gB, 5 had sIgA, and 0 had mucosal IgA. Among 12 recipients of a live HCMV vaccine, 8 had Ige to gB, 4 had sIgA, and 2 had mucosal IgA in samples collected 10-20 months after immunization; of 10 recipients of a gB vaccine, 8 had IgG to gB, 7 had sIgA, and 7 had mucosal IgA in samples collected just before or 1 month after a booster. IgG to gB and neutralizing titers in serum correlated with IgG to gB in mucosal samples. Ige to gB was in the saliva of 25 of 26 subjects with serum neutralizing titers >1:64. Serum neutralizing titers >1:64, whether induced by vaccine or wild type virus, are associated with mucosal Ige to HCMV.