AUTOMATED SYNTHESIS OF GENE FRAGMENTS
AUTOMATED SYNTHESIS OF GENE FRAGMENTS
复制标题
DOI：
10.1126/science.6169150
复制
发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
OGILVIE, KK
中科院分区：
文献类型：
--
作者：
ALVARADOURBINA, G;SATHE, GM;OGILVIE, KK
DNA/RNA合成仪为DNA序列的合成提供了一个完整的自动化程序。每个基本单元以30分钟的循环加入，允许在6 1/2小时内构建十四聚体。完整的程序进行了描述，包括所需的DNA序列的分离和纯化的实用程序。
The DNA/RNA Synthesizer provides a complete and automated procedure for the synthesis of DNA sequences. Each base unit is added in a 30-min cycle, permitting a tetradecamer to be constructed in 6 1/2 h. The complete procedure is described, including a practical procedure for isolation and purification of the desired DNA sequence.