Polysaccharides from Hedyotis diffusa enhance the antitumor activities of cytokine-induced killer cells
Polysaccharides from Hedyotis diffusa enhance the antitumor activities of cytokine-induced killer cells
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白花蛇舌草多糖增强细胞因子诱导的杀伤细胞的抗肿瘤活性
DOI：
10.1016/j.biopha.2019.109167
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发表时间：
2019
影响因子：
7.5
通讯作者：
Yang Peimin
中科院分区：
文献类型：
--
作者：
Ma Chuanjiang;Wei Yongli;Lui Qing;Xin Yizhou;Gao Guangshang;Wang Xin;Yang Peimin
白花蛇舌草是我国著名的传统中草药。从H. diffusa（HDP）具有一系列药理活性。CIK细胞输注是过继性细胞免疫治疗的一种，已成为肿瘤免疫治疗的重要手段。在本研究中，我们研究了HDP对CIK细胞的免疫刺激作用。通过培养和刺激健康志愿者的外周血单核细胞产生CIK细胞。用三种不同浓度（10、50和100 μg/mL）的HDP处理它们。通过流式细胞术检测HDP对CIK细胞群、细胞内细胞因子产生和细胞凋亡的影响。细胞毒实验检测HDP对CIK细胞的杀伤作用。此外，在小鼠模型中研究了HDP对CIK细胞的抗肿瘤活性的影响。HDP可增加CD 3 + CD 56 +CIK细胞的百分比，但对CD 4+、CD 8+或CD 4 + CD 25 +CIK细胞的百分比无显著影响。HDP处理的CIK细胞比未处理的CIK细胞显示出更强的杀伤肿瘤细胞的能力，以及更高的干扰素-γ和肿瘤坏死因子-α的产生。体外实验发现，经HDP处理的CIK细胞凋亡水平较低。此外，HDP与CIK细胞联合应用对小鼠模型中肿瘤生长的抑制作用比CIK或HDP单独治疗更强。结果表明，HDP能增强CIK细胞的抗肿瘤活性，可用于CIK细胞治疗的联合免疫治疗。
Hedyotis diffusais a well-known traditional Chinese herbal medicine. The polysaccharides extracted fromH. diffusa(HDP) exhibit a range of pharmacological activities. Transfusion of cytokine-induced killer (CIK) cell is one type of adoptive cellular immunotherapy, which is becoming an important method of cancer immunotherapy. In this present study, we investigate the immunostimulatory effect of HDP on CIK cells. CIK cells were generated by culturing and stimulating peripheral blood monocytes of healthy volunteers. They were treated with HDP at three different concentrations (10, 50, and 100 μg/mL). The effect of HDP on CIK cell populations, intracellular cytokine production, and apoptosis was examined by flow cytometry. The antitumor effect of HDP on CIK cells was determined by cytotoxicity assay. Furthermore, the effect of HDP on the antitumor activity of CIK cells in a mouse model was investigated. HDP increased the percentage of CD3+CD56+CIK cells but did not significantly change the percentage of CD4+, CD8+, or CD4+CD25+CIK cells. The HDP-treated CIK cells showed a greater ability to kill tumor cells, as well as higher production of interferon-γ and tumor necrosis factor-α, compared with the no-HDP-treated CIK cells. The HDP-treated CIK cells also found a lower apoptosis levelin vitro. Moreover, HDP combined with CIK cells had a stronger inhibitory effect on tumor growth in the mouse model compared with the CIK or HDP treatment alone. In conclusion, the results indicated that HDP enhanced the antitumor activity of CIK cells and could be used for cancer immunotherapy combined with CIK cell therapy.