MOLECULAR-CLONING AND STRUCTURE OF A PRE-B-CELL GROWTH-STIMULATING FACTOR
MOLECULAR-CLONING AND STRUCTURE OF A PRE-B-CELL GROWTH-STIMULATING FACTOR
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DOI：
10.1073/pnas.91.6.2305
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发表时间：
1994-03-15
影响因子：
11.1
通讯作者：
KISHIMOTO, T
中科院分区：
文献类型：
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作者：
NAGASAWA, T;KIKUTANI, H;KISHIMOTO, T
已知早期B细胞前体细胞的产生和增殖需要基质细胞衍生的分子。基质细胞系PA6产生一种不同于白介素7(IL-7)和干细胞因子的可溶性介质，并支持依赖基质细胞的前B细胞克隆DW34的增殖。通过表达克隆的方法，获得了该基因的编码区。该核苷酸序列包含一个由267个核苷酸组成的基本开放阅读框架，编码一个89个氨基酸的多肽。该细胞因子的氨基酸序列被命名为前B细胞生长刺激因子(Pre-B-cell Growth-刺激性因子，PBSF)，表明它是分泌α亚家族的成员。重组PBSF自身刺激了DW34细胞的增殖，并在IL-7存在的情况下协同促进了DW34细胞和骨髓B细胞前体细胞的生长。
Generation and proliferation of early B-cell progenitors have been known to require stromal cell-derived molecules. A stromal cell line, PA6, was found to produce a soluble mediator, which was distinct from interleukin 7 (IL-7) and stem cell factor and supported the proliferation of a stromal cell-dependent pre-B-cell clone, DW34. A cDNA clone encoding this DW34 growth-stimulating factor was isolated by expression cloning. The nucleotide sequence contained a single substantial open reading frame of 267 nucleotides encoding an 89-amino acid polypeptide. The amino acid sequence of this cytokine, designated pre-B-cell growth-stimulating factor (PBSF), revealed that it is a member of intercrine alpha subfamily. Recombinant PBSF stimulated the proliferation of DW34 cells for itself and, furthermore, synergistically augmented the growth of DW34 as well as bone marrow B-cell progenitors in the presence of IL-7.