Effects of silodosin on bladder activity in rats with frequent urination induced by pelvic venous congestion
Effects of silodosin on bladder activity in rats with frequent urination induced by pelvic venous congestion
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DOI：
10.1111/iju.13158
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发表时间：
2016-10-01
影响因子：
2.6
通讯作者：
Yamamoto, Hideyuki
中科院分区：
文献类型：
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作者：
Sugaya, Kimio;Nishijima, Saori;Yamamoto, Hideyuki
目的以盆腔静脉淤血致尿频雌性大鼠为实验对象，观察西洛多辛对膀胱活动的影响。方法将24只雌性大鼠分为3组：假手术组、盆腔静脉淤血组、盆腔静脉淤血/西洛多辛组。盆腔静脉充血组和盆腔静脉充血/西洛多辛组大鼠采用异氟烷麻醉，结扎双侧髂总静脉和子宫静脉。在盆腔静脉充血/西洛多辛组中，使用植入背部皮下空间的渗透泵给予西洛多辛（0.3 mg/kg/天）。 5-6周后，进行排尿行为分析、尿8-羟基脱氧鸟苷和一氧化氮代谢物测定、乌拉坦麻醉下连续膀胱测压、伊文思蓝染料膀胱外渗试验。结果与假手术大鼠相比，盆腔静脉充血大鼠排尿频率增加，尿量增加，连续膀胱测压膀胱收缩间隔缩短，尿量增加。 8-羟基脱氧鸟苷，尿一氧化氮代谢物减少，膀胱血管通透性增加。与盆腔静脉充血大鼠相比，盆腔静脉充血/西洛多辛大鼠的排尿次数减少，尿量减少，最大膀胱收缩压力较低，膀胱收缩间隔时间较长，尿中一氧化氮代谢物增加，血管通透性降低。结论西洛多辛可能改善盆腔静脉充血引起的膀胱功能障碍，也可改善盆腔静脉充血本身。
ObjectiveTo examine the effects of silodosin on bladder activity using female rats with frequent urination induced by pelvic venous congestion.MethodsA total of 24 female rats were divided into three groups: sham, pelvic venous congestion and pelvic venous congestion/silodosin group. Rats in the pelvic venous congestion and pelvic venous congestion/silodosin groups were anesthetized with isoflurane, after which the bilateral common iliac veins and uterine veins were ligated. In the pelvic venous congestion/silodosin group, silodosin (0.3 mg/kg/day) was given using an osmotic pump implanted into the subcutaneous space of the back. After 5-6 weeks, analysis of voiding behavior, measurements of urinary 8-hydroxydeoxyguanosine and nitric oxide metabolites, continuous cystometry under urethane anesthesia, and Evans blue dye extravasation test of the bladder were carried out.ResultsIn comparison with sham rats, pelvic venous congestion rats showed an increase in urination frequency with a concomitant increase in urine volume, a shorter interval between bladder contractions on continuous cystometry, an increase in urinary 8-hydroxydeoxyguanosine, a decrease in urinary nitric oxide metabolites and an increase in vesical vascular permeability. In comparison with pelvic venous congestion rats, pelvic venous congestion/silodosin rats showed a decrease in urination frequency with a concomitant decrease in urine volume, a lower maximum bladder contraction pressure, a longer interval between bladder contractions, an increase in urinary nitric oxide metabolites and a decrease in vascular permeability.ConclusionSilodosin might improve both bladder dysfunction caused by pelvic venous congestion, and the pelvic venous congestion itself.