Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
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DOI：
10.1016/j.ymben.2006.08.005
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发表时间：
2007-01-01
影响因子：
8.4
通讯作者：
van Zyl, Willem H.
中科院分区：
文献类型：
--
作者：
Den Haan, Riaan;Rose, Shaunita H.;van Zyl, Willem H.
在这项研究中，我们表达了两个纤维素酶编码基因，里氏木霉的内切葡聚糖酶（EGI）和扣囊拟青霉的β-葡萄糖苷酶（BGLI），在酿酒酵母组合。所得菌株能够通过同时产生足够的胞外内切葡聚糖酶和β-葡糖苷酶活性在磷酸溶胀纤维素（PASC）上生长。在含有10 g l（-1）PASC作为唯一碳水化合物来源的培养基上观察到厌氧生长（0.03 h（-1））高达0.27 g l（-1）DCW，同时乙醇产量高达1.0 g l（-1）。因此，我们已经证明了能够在无定形纤维素上生长并将无定形纤维素一步转化为乙醇的酵母菌株的构建，代表了在合并的生物加工配置中实现纤维素生物质一步加工的重大进展。据我们所知，这是第一次报道的重组菌株的S。酿酒酵母生长在纯纤维素上。(C)2006年爱思唯尔公司All rights reserved.
In this study, we expressed two cellulase encoding genes, an endoglucanase of Trichoderma reesei (EGI) and the beta-glucosidase of Saccharomycopsis fibuligera (BGLI), in combination in Saccharomyces cerevisiae. The resulting strain was able to grow on phosphoric acid swollen cellulose (PASC) through simultaneous production of sufficient extracellular endoglucanase and beta-glucosidase activity. Anaerobic growth (0.03 h(-1)) up to 0.27 g l(-1) DCW was observed on medium containing 10 g l(-1) PASC as sole carbohydrate source with concomitant ethanol production of up to 1.0 g l(-1). We have thus demonstrated the construction of a yeast strain capable of growth on and one-step conversion of amorphous cellulose to ethanol, representing significant progress towards realization of one-step processing of cellulosic biomass in a consolidated bioprocessing configuration. To our knowledge, this is the first report of a recombinant strain of S. cerevisiae growing on pure cellulose. (C) 2006 Elsevier Inc. All rights reserved.