Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1
Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B1
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乳胶凝集抑制反应试验快速检测黄曲霉毒素B1的评价
DOI：
10.1016/j.foodcont.2011.01.003
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发表时间：
2011-07
期刊：
影响因子：
6
通讯作者：
Fusheng Chen
中科院分区：
文献类型：
--
作者：
Xiaohong Wang;Ping Wang;Youxiang Zhou;Yang Ye;Fusheng Chen
黄曲霉毒素B1(AFB1)是一种霉菌毒素，主要由某些曲霉属物种产生，广泛存在于多种农产品中。为了避免 AFB1 消费的风险，需要对许多农产品、食品和饲料进行分析，并且需要一种快速且非仪器的方法来检测 AFB1。在本研究中，建立了一种快速、廉价且用户友好的乳胶凝集抑制反应试验（LAIRT），用于现场检测 AFB1。首先，通过无皂聚合制备羧基聚苯乙烯乳胶颗粒（CPLP），并用黄曲霉毒素B1肟-BSA（AFB1O-BSA）敏化用于检测AFB1。在 LAIRT 中，5 ng/mL AFB1 抑制了 AFB1O-BSA 致敏 CPLP 和抗 AFB1 抗血清混合物的凝集反应，并且一张载玻片上 6 个样品的分析时间少于 10 分钟。随后，对10份大米和花生样品进行LAIRT和ELISA分析，结果显示1份大米样品和2份花生样品呈阳性，与ELISA结果一致。然而，LAIRT 比 ELISA 可以更快地获得结果。这种简单快速的 LAIRT 可能有助于在田间筛选农产品、食品和饲料的 AFB1。
Aflatoxin B1(AFB1), a mycotoxin mainly produced by some Aspergillus species, has been found in a wide range of agricultural products. To avoid the risk of AFB1consumption, many agricultural commodities, foods and feeds should be analyzed and a rapid and non-instrumental method for the detection of AFB1is needed. In this study, a rapid, inexpensive and user-friendly latex agglutination inhibition reaction test (LAIRT) for on site testing of AFB1had been established. At first, carboxylated polystyrene latex particles (CPLP) were prepared by soap-free polymerization and sensitized with aflatoxin B1oxime-BSA (AFB1O-BSA) for the detection of AFB1. In LAIRT, the agglutination reaction with AFB1O-BSA-sensitized CPLP and anti-AFB1antiserum mixture was inhibited by 5 ng/mL AFB1and the analysis time for 6 samples on one glass slide was less than 10 min. Subsequently, 10 rice and peanut samples were analyzed by LAIRT and ELISA, and the results showed that 1 rice sample and 2 peanut samples were positive and in agreement with those of ELISA. However, the results could be obtained more rapidly by LAIRT than ELISA. This easy and rapid LAIRT might be useful for screening AFB1of agricultural commodities, foods and feeds in the field.
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影响因子：
2.2
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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DOI：
10.1016/s0927-7757(01)01033-0
发表时间：
2002-03
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects
影响因子：
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作者：
Chen Lu;R. Pelton
通讯作者：
Chen Lu;R. Pelton