FORMATION OF AN ADENINE-THYMINE PHOTOADDUCT IN THE DEOXYDINUCLEOSIDE MONOPHOSPHATE D(TPA) AND IN DNA
FORMATION OF AN ADENINE-THYMINE PHOTOADDUCT IN THE DEOXYDINUCLEOSIDE MONOPHOSPHATE D(TPA) AND IN DNA
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DOI：
10.1126/science.6836308
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
MCCLOSKEY, JA
中科院分区：
文献类型：
--
作者：
BOSE, SN;DAVIES, RJH;MCCLOSKEY, JA
在254 nm波长下，脱氧二核苷单磷酸d（TpA）的A和T碱基在水溶液中形成光加合物。用酸处理光加合物将其特异性地转化为荧光水解产物C7 H7 N3 O，其结合了A的8位C和T的甲基。从寡聚和多聚脱氧核糖核苷酸的酸水解产物中分离荧光水解产物表明，光加合物是由d（pTpA）、d（TpApT）、d（TpApTpA）、poly（dA-dT）以及单链和双链DNA的UV照射形成的。
A photoadduct is formed between the A and T bases of the deoxydinucleoside monophosphate d(TpA) when it is irradiated at 254 nm in aqueous solution. Treatment of the photoadduct with acid converts it specifically into a fluorescent hydrolysis product, C7H7N3O, incorporating the position-8 C of A and the methyl group of T. Isolation of the fluorescent hydrolysis product from acid hydrolyzates of oligo- and polydeoxyribonucleotides shows that the photoadduct is formed by UV irradiation of d(pTpA), d(TpApT), d(TpApTpA), poly(dA-dT), and both single and double stranded DNA.