Profiling analysis of amino acids from hyperlipidaemic rats treated with Gynostemma pentaphyllum and atorvastatin
Profiling analysis of amino acids from hyperlipidaemic rats treated with Gynostemma pentaphyllum and atorvastatin
复制标题
绞股蓝和阿托伐他汀治疗高脂血症大鼠的氨基酸谱分析
DOI：
10.3109/13880209.2016.1152278
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发表时间：
2016-03
影响因子：
3.8
通讯作者：
Chunjie Zhao
中科院分区：
文献类型：
--
作者：
Yinan Wang;Min Zhao;Miao Wang;Chunjie Zhao
摘要 背景绞股蓝在传统医学中用于治疗高脂血症已有悠久的历史。目的：本研究的目的是评价绞股蓝（GP）和阿托伐他汀对高脂血症大鼠血浆和肝组织氨基酸的影响。材料与方法 大鼠连续高脂饮食喂养11周，构建高脂血症模型。高脂血症大鼠给予绞股蓝(120mg/kg)和阿托伐他汀(1.8mg/kg)治疗4周,每天灌胃1次。色谱分离在Shim-pack XR-ODSIII C18分析柱(75mm×2.0mm内径，1.6μm，Shmadazu Corp.，东京，日本)上进行。通过主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）鉴定氨基酸的生物标志物。结果 高脂血症模型大鼠喂食高脂饲料后，TC和LDL-C值显着升高（p<0.01）。所建立的方法可以对 12 种氨基酸进行目标分析。 PCA研究表明，GP治疗后血浆氨基酸尚未恢复正常，但阿托伐他汀治疗后血浆氨基酸略有恢复。 GP对氨基酸代谢几乎没有影响，而阿托伐他汀可以通过自我调节机制改变氨基酸代谢。讨论与结论UPLC/DAD 与SCX-SPE 结合可以成功地用于氨基酸的图谱分析。通过比较GP和阿托伐他汀治疗后的生物标志物，揭示两种药物对生物标志物的影响。
Abstract Context Gynostemma pentaphyllum (Thunb.) Makino has been used in traditional medicine for the treatment of hyperlipidaemic with a long history. Objective: The objective of this study was to evaluate the influence of Gynostemma pentaphyllum (GP) and atorvastatin on amino acids from the plasma and liver tissue of hyperlipidaemic rats. Materials and methods The rats were fed a high-fat diet continuously for 11 weeks for the construction of hyperlipidaemic model. The hyperlipidaemic rats were treated with Gynostemma pentaphyllum (120 mg/kg) and atorvastatin (1.8 mg/kg) for 4 weeks, and the rats were intragastric administration one time every day. Chromatographic separation was performed on a Shim-pack XR-ODSIII C18 analytical column (75 mm × 2.0 mm i.d., 1.6 μm, Shmadazu Corp., Tokyo, Japan). The biomarkers of amino acids were identified by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). Results After feeding with a high-fat diet, the TC and LDL-C values of the hyperlipidaemic mode rats increased dramatically (p < 0.01). The established method allowed a target analysis of 12 kinds of amino acids. PCA studies showed that the plasma amino acids had not returned to normal after GP treatment, but those had recovered slightly after atorvastatin treatment. GP has almost no impact on the metabolism of amino acids, while atorvastatin can modify the metabolism of amino acids via self-regulatory mechanisms. Discussion and conclusion UPLC/DAD combined with SCX-SPE can be successfully used for profiling analysis of amino acids. By the comparison of biomarkers following treatment with GP and atorvastatin, the influence of the two drugs on biomarkers is revealed.
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DOI：
10.3736/jcim20040201
发表时间：
2004-03
期刊：
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
影响因子：
--
作者：
Hao-zhu Chen
通讯作者：
Hao-zhu Chen
DOI：
10.4158/ep.18.s1.1
发表时间：
2012-04
期刊：
Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists
影响因子：
--
作者：
P. Jellinger;Donald A. Smith;A. Mehta;O. Ganda;Y. Handelsman;H. Rodbard;M. Shepherd;J. Seibel
通讯作者：
P. Jellinger;Donald A. Smith;A. Mehta;O. Ganda;Y. Handelsman;H. Rodbard;M. Shepherd;J. Seibel
影响因子：
5.4
作者：
S. Grover;M. Dorais;L. Coupal
通讯作者：
S. Grover;M. Dorais;L. Coupal
影响因子：
7.4
作者：
Chang Wang;Hongwei Kong;Yufeng Guan;Jun Yang;J. Gu;Shengli Yang;Guowang Xu
通讯作者：
Chang Wang;Hongwei Kong;Yufeng Guan;Jun Yang;J. Gu;Shengli Yang;Guowang Xu
影响因子：
7.4
作者：
K. Sonkar;Rudra N Purusottam;N. Sinha
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K. Sonkar;Rudra N Purusottam;N. Sinha