Mouse Genome Editing Using the CRISPR/Cas System.
Mouse Genome Editing Using the CRISPR/Cas System.
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DOI：
10.1002/0471142905.hg1507s83
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发表时间：
2014-10-01
影响因子：
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通讯作者：
Gurumurthy CB
中科院分区：
文献类型：
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作者：
Harms DW;Quadros RM;Seruggia D;Ohtsuka M;Takahashi G;Montoliu L;Gurumurthy CB
产生所需基因突变的技术的可用性使实验室小鼠成为生物医学研究（包括人类遗传学）中广泛使用的模式生物。这个现有技术库的新成员是CRISPR/Cas系统。具体而言，该系统允许比以前使用的技术更快地编辑小鼠基因组，更重要的是，可以在单个实验中创建多个突变。在这里，我们提供了制备CRISPR/Cas试剂和显微注射到单细胞小鼠胚胎中以创建敲除或敲入小鼠模型的方案。
The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much faster than the previously used techniques and more importantly multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one cell mouse embryos to create knockout or knock-in mouse models.