Insulin-like peptides in the mosquito Anopheles stephensi: identification and expression in response to diet and infection with Plasmodium falciparum.
Insulin-like peptides in the mosquito Anopheles stephensi: identification and expression in response to diet and infection with Plasmodium falciparum.
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DOI：
10.1016/j.ygcen.2011.06.005
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Luckhart, Shirley
中科院分区：
文献类型：
--
作者：
Marquez, Alexander G.;Pietri, Jose E.;Smithers, Hannah M.;Nuss, Andrew;Antonova, Yevgeniya;Drexler, Anna L.;Riehle, Michael A.;Brown, Mark R.;Luckhart, Shirley
胰岛素样肽(ILPS)调节多种生物过程，包括新陈代谢和对感染的免疫，并在广泛不同的类群中共享相似的结构基序。胰岛素/胰岛素样生长因子信号通路(IIS)元件也同样保守。我们已经证明IIS调节雌性斯氏按蚊的生殖、先天免疫和寿命，斯氏按蚊是人类疟疾的主要蚊媒。为了进一步探索IIS对这些过程的调控，我们在该物种中发现了编码五个ILPS的基因，并表征了它们在组织中的表达。用冈比亚按蚊ILP同源物的抗血清鉴定其细胞来源。通过免疫细胞化学检测雌性斯氏杆菌。我们用定量逆转录-聚合酶链式反应分析了不同喂养方式和恶性疟原虫感染后，年轻和老年雌性个体中ILP的组织特异性转录表达。虽然一些ILP转录本的变化在老年女性和对血液喂养的反应中是明显的，但在对摄入的人胰岛素的激素浓度和对恶性疟原虫感染的反应中尤其显著。这些变化表明，ILP的分泌和作用在感染疟原虫的雌性体内可能也有类似的反应，并可能改变新陈代谢和先天免疫。
Insulin-like peptides (ILPs) regulate a multitude of biological processes, including metabolism and immunity to infection, and share similar structural motifs across widely divergent taxa. Insulin/insulin-like growth factor signaling (IIS) pathway elements are similarly conserved. We have shown that IIS regulates reproduction, innate immunity, and lifespan in female Anopheles stephensi, a major mosquito vector of human malaria. To further explore IIS regulation of these processes, we identified genes encoding five ILPs in this species and characterized their expression in tissues. Antisera to ILP homologs in Anopheles gambiae were used to identify cellular sources in An. stephensi females by immunocytochemistry. We analyzed tissue-specific ILP transcript expression in young and older females, in response to different feeding regimens, and in response to infection with Plasmodium falciparum with quantitative reverse transcriptase-PCR assays. While some ILP transcript changes were evident in older females and in response to blood feeding, significant changes were particularly notable in response to hormonal concentrations of ingested human insulin and to P. falciparum infection. These changes suggest that ILP secretion and action may be similarly responsive in Plasmodium-infected females and potentially alter metabolism and innate immunity.
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影响因子：
3.1
作者：
Lim, JH;Gowda, DC;Luckhart, S
通讯作者：
Luckhart, S
影响因子：
2.2
作者：
QURAISHI, MS;FAGHIH, MA;ESGHI, N
通讯作者：
ESGHI, N
影响因子：
9.6
作者：
Drexler, Anna L.;Vodovotz, Yoram;Luckhart, Shirley
通讯作者：
Luckhart, Shirley
DOI：
10.1073/pnas.0800478105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Brown, Mark R.;Clark, Kevin D.;Strand, Michael R.
通讯作者：
Strand, Michael R.
影响因子：
3
作者：
Riehle, Michael A.;Fan, Yongliang;Brown, Mark R.
通讯作者：
Brown, Mark R.