Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii)
Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii)
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DOI：
10.1016/j.aquaculture.2016.05.011
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发表时间：
2017-09
期刊：
影响因子：
4.5
通讯作者：
H. Yoshikawa;Y. Takeuchi;Yasuko Ino;Junjie Wang;G. Iwata;N. Kabeya;R. Yazawa;G. Yoshizaki
中科院分区：
文献类型：
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作者：
H. Yoshikawa;Y. Takeuchi;Yasuko Ino;Junjie Wang;G. Iwata;N. Kabeya;R. Yazawa;G. Yoshizaki
精原细胞腹腔内移植在商业化水产养殖中从替代亲鱼生产异源或异种供体衍生的后代中可能变得重要。然而，与嵌合生殖系的代理人产生和贡献捐助者衍生的配子和后代的后代的速度必须提高这项技术变得实用，特别是对海洋鱼类。在这里，我们进行了腹腔内精原细胞移植到三倍体黄姑鱼mitsukurii评估功能不育的三倍体作为替代品的适用性。从半合子pHSC-GFP转基因（gfp/−）个体中收集供体睾丸细胞，然后移植到12日龄幼虫（二倍体或三倍体）的腹膜腔中。移植后6个月，37%（男性）和29%（女性）的三倍体受体产生了gfp阳性的供体来源的配子;这些比率分别比二倍体男性和女性受体的比率高7倍和4倍。来自三倍体受体的精子和卵子分别与来自野生型鱼类的卵子或精子人工受精;大约50%的后代是GFP阳性的，这表明几乎所有的后代都来自供体来源的配子，这些配子来自移植的GFP/−生殖细胞。替代三倍体受体的繁殖力和产卵频率与非移植二倍体受体没有显著差异，并且三倍体受体至少在两个产卵季节产生随后的供体衍生后代。我们的研究结果表明，使用功能不育的三倍体受体提高了产生供体来源的配子的受体的百分比。此外，三倍体受体的使用导致在这个海洋硬骨鱼的供体来源的配子的独家生产。声明的relevanceIntra-peritoneal精原细胞移植使用异源三倍体受体首次证明在海洋硬骨鱼。除了有效和排他性的供体来源的配子发生，我们发现使用功能不育的三倍体受体提高了产生供体来源的配子的受体的百分比。因此，三倍体替代品比二倍体替代品更合适，使用三倍体替代品可能会加速替代亲鱼技术在水产养殖目标，遗传价值或濒危海洋硬骨鱼类中的应用。
Intra-peritoneal spermatogonial transplantation could become important in the production of allogenic or xenogenic donor-derived offspring from surrogate parent fish in commercial aquaculture. However, the rates at which surrogates with chimeric germ-lines are produced and contribute donor-derived gametes and offspring to subsequent generations must be improved for this technology to become practical, especially for marine fishes. Here, we performed intra-peritoneal spermatogonial transplantation into triploid Nibe croakerNibea mitsukuriito evaluate the suitability of functionally sterile triploids as surrogates. Donor testicular cells were collected from hemizygous pHSC-GFP transgenic (gfp/−) individuals and then transplanted into the peritoneal cavities of 12-day-old larvae (diploid or triploid). By 6 months after transplantation, 37% (male) and 29% (female) of triploid recipients had producedgfp-positive, donor-derived gametes; these rates were 7- and 4-fold higher, respectively, than the rates for diploid male and female recipients, respectively. Sperm and eggs from triploid recipients were artificially fertilized with eggs or sperm, respectively, from wild-type fish; approximately 50% of the resulting offspring weregfp-positive, suggesting that nearly all of offspring originated from donor-derived gametes that had descended from transplantedgfp/− germ cells. The fecundity and spawning frequency of surrogate triploid recipients were not significantly different to those of non-transplanted diploids, and the triploid recipients produced subsequent donor-derived offspring for at least two spawning seasons. Our results demonstrated that use of functionally sterile triploid recipients improved the percentage of recipients that produce donor-derived gametes. Further, the use of triploid recipients resulted in exclusive production of donor-derived gametes in this marine teleost.Statement of relevanceIntra-peritoneal spermatogonial transplantation using allogenic triploid recipients was first demonstrated in marine teleosts. In addition to efficient and exclusive donor-derived gametogenesis, we found that use of functionally sterile triploid recipients improved the percentage of recipients that produce donor-derived gametes. Consequently, triploid surrogates were more suitable than diploid surrogates and that use of triploid surrogates may accelerate the application of surrogate broodstock technology in aquaculture-targeted, genetically valuable, or endangered marine teleosts.