Time Course of Metabolic Alterations Associated with the Progression of Systemic Lupus Erythematosus in MRL/lpr Mice Based on GC/MS
Time Course of Metabolic Alterations Associated with the Progression of Systemic Lupus Erythematosus in MRL/lpr Mice Based on GC/MS
复制标题
基于 GC/MS 的 MRL/lpr 小鼠系统性红斑狼疮进展相关代谢变化的时间进程
DOI：
10.1021/acs.jproteome.0c00619
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发表时间：
2021
影响因子：
4.4
通讯作者：
Jia Zhou
中科院分区：
文献类型：
--
作者：
Jiajia Liu;Dingyi Zhang;Keer Wang;Zhengfu Li;Zhaochun He;Dehong Wu;Zhenghao Xu;Jia Zhou
探究疾病发展过程中代谢产物和代谢途径的动态变化，有助于进一步了解系统性红斑狼疮（SLE）的病因和发病机制。在这项研究中，采用基于气相色谱/质谱（GC/MS）的血清代谢组学研究了9、11和13周龄狼疮易感小鼠（MRL/lpr）在SLE不同阶段的代谢变化。进行多变量统计分析以查看 MRL/lpr 小鼠和年龄匹配的 C57BL/6 小鼠之间代谢谱的变化，并使用 t 检验和倍数变化标准来识别每个阶段的差异代谢物。 9周龄时MRL/lpr小鼠中发现11种代谢物发生变化，主要涉及三羧酸（TCA）循环、糖酵解和丁酸代谢；随着周龄的增加，TCA循环仍受到干扰，脂肪酸生物合成自11周龄起显着上调；此外，尿素、尿酸盐和3-吲哚乳酸盐在13周龄时有所增加。我们发现 MRL/lpr 小鼠代谢改变的时间过程，这可能与 SLE 的进展有关。这些发现可为研究SLE的发病机制、判断疾病的病理阶段和严重程度提供参考。 MS 数据已存入 Mendeley (https://www.mendeley.com/)。
Exploring the dynamic changes of metabolites and metabolic pathways during the development of the disease can help to further understand the etiology and pathogenesis of systemic lupus erythematosus (SLE). In this study, serum metabolomics based on gas chromatography/mass spectrometry (GC/MS) was employed to investigate the metabolic alterations at different stages of SLE using lupus-prone mice (MRL/lpr) of 9, 11, and 13 weeks of age. Multivariate statistical analysis was performed to view the alterations of metabolic profiles between MRL/lpr mice and age-matched C57BL/6 mice, andt-test and fold change criteria were used to identify differential metabolites at each stage. 11 changed metabolites were found in MRL/lpr mice at 9 weeks of age, which were mainly involved in the tricarboxylic acid (TCA) cycle, glycolysis, and butanoate metabolism; with the increase of week age, the TCA cycle was still disturbed, and the biosynthesis of fatty acids was significantly upregulated since 11 weeks of age; in addition, urea, urate, and indole-3-lactate were increased at 13 weeks of age. We found a time course of metabolic alterations in MRL/lpr mice, which may be related to the progression of SLE. These findings could provide a reference for studying the mechanism of SLE and judging the pathological stage and severity of the disease. The MS data have been deposited in Mendeley (https://www.mendeley.com/).