Pancreatic duct obstruction itself induces expression of a smooth muscle actin in pancreatic stellate cells
Pancreatic duct obstruction itself induces expression of a smooth muscle actin in pancreatic stellate cells
复制标题
DOI：
10.1016/s0022-4804(03)00153-7
复制
发表时间：
2003-09-01
影响因子：
2.2
通讯作者：
Yokozaki, H
中科院分区：
文献类型：
--
作者：
Kishi, S;Takeyama, Y;Yokozaki, H
背景资料。胰腺星状细胞(PSCs)被认为是导致胰腺纤维化的原因。尽管纤维化是胰管梗阻所致慢性胰腺炎(CP)的主要特征，但目前尚不清楚胰管梗阻本身是否会激活PSC。为了验证胰管梗阻激活PSCs的假设，临床和实验分析使用了平滑肌肌动蛋白(α-SMA)作为PSCs激活的标志。在临床分析中，将胰腺癌和乳头状癌患者的手术标本分为有或无胆管梗阻两组。检测这些标本上α-SMA的表达，并评价两组之间的差异。在动物实验中，通过结扎十二指肠段次级胰管建立大鼠胰腺炎模型，并检测α-SMA的表达。在临床分析中，有胆管梗阻的胰腺组织(14例)的α-SMA表达明显强于无胆管梗阻的胰腺组织(7例)。前者所有标本均有α-SMA表达，而后者有4例标本完全不表达(P<；0.05)。在动物实验中，α-SMA在结扎后7天即可检测到表达，第10天表达增强。我们可以假设胰管梗阻本身会激活PSCs。这一机制可能在脑性瘫痪的发生发展过程中起着多方面的作用。(C)2003 Elsevier Inc.保留所有权利。
Background. Pancreatic stellate cells (PSCs) are thought to be responsible for pancreatic fibrosis. Although fibrosis is a major characteristic of chronic pancreatitis (CP) induced by pancreatic duct obstruction, it is unclear whether pancreatic duct obstruction itself activates PSCs.Materials and methods. To test the hypothesis that pancreatic duct obstruction activates PSCs, clinical and experimental analyses were performed using a smooth muscle actin (alpha-SMA) as a marker of their activation. In clinical analysis, surgical specimens from the patients with pancreatic cancer or cancer of the papilla Vater were classified into two groups with or without duct obstruction. alpha-SMA expression was examined on these specimens, and the difference between two groups was evaluated. In animal experiment, duct ligation-induced pancreatitis was developed in rats by ligating the secondary pancreatic duct in duodenal segment, and the expression of alpha-SMA was examined.Results. In clinical analysis, the specimens from the pancreas with duct obstruction (14 cases) expressed alpha-SMA significantly stronger than those from the pancreas without duct obstruction (7 cases). All specimens in the former expressed alpha-SMA, but 4 specimens from the latter did not at all (P < 0.05). In animal experiment, alpha-SMA expression was detected 7 days after the ligation and was increased on the 10th day.Conclusions. We can assume that pancreatic duct obstruction itself activates PSCs. This mechanism may play roles in the development of CP from multiple origins. (C) 2003 Elsevier Inc. All rights reserved.