Phenotypic and molecular characterization of community occurring, Western Samoan phage pattern methicillin-resistant Staphylococcus aureus
Phenotypic and molecular characterization of community occurring, Western Samoan phage pattern methicillin-resistant Staphylococcus aureus
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DOI：
10.1093/jac/dkf242
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发表时间：
2002-12-01
影响因子：
5.2
通讯作者：
Smith, JMB
中科院分区：
文献类型：
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作者：
Adhikari, RP;Cook, GM;Smith, JMB
在新西兰，据估计，从患者身上回收的耐甲氧西林金黄色葡萄球菌（MRSA）菌株中有一半以上属于所谓的西萨摩亚噬菌体模式1型和2型（WSPP 1、WSPP 2）。这些菌株与经典MRSA分离株的不同之处在于它们缺乏多重耐药性和社区发生，这表明这些菌株具有不同于其他MRSA的性质和/或特征。为了解决这一假设，将来自西萨摩亚、新西兰和澳大利亚的10株WSPP 1和WSPP 2分离株与常见的医院MRSA分离株进行了比较。所有WSPP分离物的脉冲场凝胶电泳模式的SmaI消化的DNA，凝固酶基因限制性片段长度多态性模式和本地化的194 kb的SmaI消化片段的mecA是相同的。与医院流行的MRSA菌株相比，WSPP菌株对各种环境应力（例如皮肤脂肪酸，紫外线，干燥）没有更多的抗性/敏感性，除了它们对盐的更高耐受性。在毒力方面，WSPP MRSA在数量上更好地附着在上皮细胞系HEp 2上，与非WSPP MRSA分离株相比，均为蛋黄混浊因子阴性，并产生更高水平的溶血毒素。
In New Zealand, it is estimated that greater than half of the methicillin-resistant Staphylococcus aureus (MRSA) strains recovered from patients belong to what has been termed Western Samoan phage pattern types 1 and 2 (WSPP1, WSPP2). These strains differ from classical MRSA isolates in terms of their lack of multiresistance and community occurrence, suggesting that such strains possess properties and/or characteristics different from those of other MRSA. To address this hypothesis, 10 WSPP1 and WSPP2 isolates from Western Samoa, New Zealand and Australia were compared with common hospital MRSA isolates. All WSPP isolates were identical with regard to pulsed-field gel electrophoretic pattern of SmaI-digested DNA, coagulase gene restriction fragment length polymorphism pattern and localization of mecA to a 194 kb SmaI digestion fragment. The WSPP strains were no more resistant/sensitive to various environmental stresses (e.g. skin fatty acids, UV light, desiccation) compared with hospital epidemic MRSA strains, except for their higher tolerance to salt. In terms of virulence, the WSPP MRSA were quantitatively better at attaching to the epithelial cell line HEp2, were uniformly egg-yolk opacity factor negative and produced higher levels of haemolytic toxins compared with non-WSPP MRSA isolates.