Responses from two firing patterns in inferior colliculus neurons to stimulation of the lateral lemniscus dorsal nucleus.
Responses from two firing patterns in inferior colliculus neurons to stimulation of the lateral lemniscus dorsal nucleus.
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下丘神经元的两种放电模式对外侧丘系背核刺激的反应
DOI：
10.4103/1673-5374.182706
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发表时间：
2016-05
影响因子：
6.1
通讯作者：
Zhao JY
中科院分区：
文献类型：
--
作者：
Li XT;Wang NY;Wang YJ;Xu ZQ;Liu JF;Bai YF;Dai JS;Zhao JY
下丘的γ-氨基丁酸能神经元（GABA能神经元）根据其对恒定电流注入的固有电特性分为各种类型。虽然这种分类与生理功能有关，但对于具有各种放电模式的神经元在声学处理中的确切作用仍然知之甚少。在本研究中，我们分析了下丘神经元在体外的特性，并记录反应的刺激外侧丘系背核的全细胞膜片钳技术。7个下丘神经元被测试，并分为两种放电模式：持续规则（n = 4）和持续适应放电模式（n = 3）。大多数下丘神经元对刺激和荷包牡丹碱的反应略有变化。一个具有持续适应放电模式的神经元的反应在刺激后被抑制，但在应用γ-氨基丁酸受体拮抗剂后恢复到正常水平。一个神经元的持续规则模式显示抑制刺激反应，这是不受荷包牡丹碱。结果提示，下丘GABA能神经元具有持续规则或持续适应的放电模式。此外，从外侧丘系背核到下丘的GABA能投射与声音定位有关。不同的神经元反应的各种放电模式表明在声音定位的作用。更好地理解这些机制和功能将为听力缺陷提供更好的临床治疗模式。
The γ-aminobutyric acid neurons (GABAergic neurons) in the inferior colliculus are classified into various patterns based on their intrinsic electrical properties to a constant current injection. Although this classification is associated with physiological function, the exact role for neurons with various firing patterns in acoustic processing remains poorly understood. In the present study, we analyzed characteristics of inferior colliculus neurons in vitro, and recorded responses to stimulation of the dorsal nucleus of the lateral lemniscus using the whole-cell patch clamp technique. Seven inferior colliculus neurons were tested and were classified into two firing patterns: sustained-regular (n = 4) and sustained-adapting firing patterns (n = 3). The majority of inferior colliculus neurons exhibited slight changes in response to stimulation and bicuculline. The responses of one neuron with a sustained-adapting firing pattern were suppressed after stimulation, but recovered to normal levels following application of the γ-aminobutyric acid receptor antagonist. One neuron with a sustained-regular pattern showed suppressed stimulation responses, which were not affected by bicuculline. Results suggest that GABAergic neurons in the inferior colliculus exhibit sustained-regular or sustained-adapting firing patterns. Additionally, GABAergic projections from the dorsal nucleus of the lateral lemniscus to the inferior colliculus are associated with sound localization. The different neuronal responses of various firing patterns suggest a role in sound localization. A better understanding of these mechanisms and functions will provide better clinical treatment paradigms for hearing deficiencies.