Autofluorescence distribution associated with drusen in age-related macular degeneration.
Autofluorescence distribution associated with drusen in age-related macular degeneration.
复制标题
DOI：
--
复制
发表时间：
2000-02
影响因子：
4.4
通讯作者：
F. Delori;M. Fleckner;D. Goger;J. Weiter;C. Dorey
中科院分区：
文献类型：
--
作者：
F. Delori;M. Fleckner;D. Goger;J. Weiter;C. Dorey
目的：探讨年龄相关性黄斑病变和黄斑变性（ARM/AMD）患者的drusen是否与视网膜色素上皮（RPE）脂褐素荧光的局灶性改变有关。方法采用一种新型的自体荧光成像装置，对20例ARM/AMD患者的脂褐素分布与个体dren的相关性进行研究。使用配对的单色和自体荧光眼底图像详细分析了含有drusen的特定部位的自体荧光地形。在4只眼中，通过图像分析比较了自身荧光的空间分布与样本的位置，并量化了个体样本（54只样本）上的自身荧光分布。结果。一种特定的自体荧光模式经常被发现在空间上与60至175微米大小的硬瘤和软瘤相关。这种模式的特征是中心区域的自体荧光减少，在大多数情况下，周围是一个环的自体荧光增加。该模式的位置与不同个体的位置高度相关。中心低自身荧光焦点平均比周围背景低16%，而环，当存在时，平均比背景高6%的荧光。大于175微米的软瘤和融合软瘤要么表现为低自身荧光的多焦点区域，要么表现为更不均匀的分布。结论。自体荧光成像允许在特定部位测量RPE脂褐素。RPE上覆的患者自身荧光发生改变，提示RPE健康状况发生改变。
PURPOSE To determine whether drusen in patients with age-related maculopathy and macular degeneration (ARM/AMD) are associated with focal changes in retinal pigment epithelium (RPE) lipofuscin fluorescence. METHOD A new autofluorescence imaging device was used to study lipofuscin distribution associated with individual drusen in 20 patients with ARM/AMD. Paired monochromatic and autofluorescence fundus images were used for detailed analysis of the topography of autofluorescence at specific sites containing drusen. In four eyes, image analysis was used to compare the spatial distribution of the autofluorescence with the location of drusen and to quantify the autofluorescence distribution over individual drusen (54 drusen). REsuLTs. A specific pattern of autofluorescence was frequently found to be spatially associated with hard drusen and soft drusen between 60 and 175 microm in size. The pattern is characterized by a central area of decreased autofluorescence surrounded, in most cases, by an annulus of increased autofluorescence. The location of this pattern was highly correlated with the position of individual distinct drusen. The central low autofluorescence focus was on average 16% below the surrounding background, and the annulus, when present, was on average 6% more fluorescent than the background. Soft drusen larger than 175 microm and confluent soft drusen show either multifocal areas of low autofluorescence or a more heterogeneous distribution. CONCLUSIoNs. Autofluorescence imaging permits measurement of RPE lipofuscin at specific sites. RPE overlying drusen have altered autofluorescence, suggesting changes in RPE health.