Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors
Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors
复制标题
两种合成HNK-1碳水化合物表位的葡萄糖醛酸转移酶在大鼠肌源性祖细胞亚系中的差异表达
DOI：
10.1016/s0925-4773(00)00449-4
复制
发表时间：
2000
影响因子：
2.6
通讯作者：
N. Osumi
中科院分区：
文献类型：
--
作者：
T. Nagase;Y. Shimoda;Y. Sanai;Shun;K. Harii;N. Osumi
HNK-1表位是介导各种细胞-细胞或细胞-底物相互作用的细胞表面碳水化合物。我们发现HNK-1表位在轴上肌节亚群的纵向排列纤维中，以及轴下成肌细胞迁移到大鼠胚胎的肢芽中。我们接下来研究了编码HNK-1表位生物合成所需的两种葡萄糖醛酸转移酶（GlcAT-P、GlcAT-D）和磺基转移酶（Sul-T）的基因的表达模式。GlcAT-P基因在非迁移性纵行肌纤维中表达，GlcAT-D基因在迁移性成肌细胞中表达。Sul-T基因表达在所有这些肌源性人群中普遍存在。因此，GlcAT基因的差异表达可能与轴上/轴下或迁移/非迁移成肌细胞谱系有关。
HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages.