Influx of L-arginine is an indicator of membrane potential in human fibroblasts.
Influx of L-arginine is an indicator of membrane potential in human fibroblasts.
复制标题
L-精氨酸的流入是人成纤维细胞膜电位的指标。
DOI：
--
复制
发表时间：
1989
影响因子：
--
通讯作者：
G. Gazzola
中科院分区：
文献类型：
--
作者：
O. Bussolati;P. C. Laris;F. Nucci;V. Dall’Asta;R. Franchi‐Gazzola;G. Guidotti;G. Gazzola
采用L-精氨酸（JARG）的净流入作为人成纤维细胞中膜电位的指标。通过增加[K+]输出获得的细胞去极化降低了JARG和脂溶性阳离子四苯基磷（JTPP）（膜电位探针）的净流入。JTPP，但不是JARG，线粒体电位的影响，并表现出依赖于细胞内和/或细胞外结合的组件。JARG对L-脯氨酸的Na+依赖性转运或Na+-K+-ATP酶活性引起的膜电位变化敏感。在50 μ M缬氨霉素存在下，在[K +]out为1.5 ~ 100 mM范围内，JARG受K+分配比的显著影响。在[K +]out为1.5 ~ 100 mM范围内，膜电位（Em）在-90 ~-23 mV之间变化，JARG与膜电位的校准产生线性关系。这些结果表明：（1）TPP+的净内流不是培养的人成纤维细胞膜电位的可靠指标，（2）L-精氨酸的净内流可作为同一细胞膜电位的指标;（3）在Em为-23 ~-90 mV范围内，系统y+的活度（在培养的人成纤维细胞中专门用于L-精氨酸转运的膜机构）没有表现出转运的电位依赖性活化的饱和。
The net influx of L-arginine (JARG) was employed as an indicator of the membrane potential in human fibroblasts. Cell depolarization, obtained by increasing [K+]out, decreased both JARG and the net influx of the lipid soluble cation tetraphenylphosphonium (JTPP), a probe of membrane potential. JTPP, but not JARG, was influenced by the mitochondrial potential and exhibited a component dependent on intracellular and/or extracellular binding. JARG was sensitive to changes in the membrane potential induced by Na+-dependent transport of L-proline or by the activity of Na+-K+-ATPase. In the presence of 50 microM valinomycin, JARG was markedly influenced by the distribution ratio of K+ in a range of [K+]out from 1.5 to 100 mM. In this range of [K+]out, membrane potential (Em) varied from -90 to -23 mV, and calibration of JARG vs. the membrane potential yielded a linear relationship. These results indicate the following: 1) that the net influx of TPP+ is not a reliable indicator of membrane potential in cultured human fibroblasts; 2) that in the same cells the net influx of L-arginine can be employed as an index of membrane potential; 3) that in a range of Em from -23 to -90 mV the activity of system y+ (the membrane agency devoted to L-arginine transport in cultured human fibroblasts) exhibits no saturation of potential-dependent activation of transport.
兔肾刷状缘膜囊泡中不依赖 Na 的 L-精氨酸转运。
DOI：
10.1016/0005-2736(82)90036-0
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hammerman,MR
通讯作者：
Hammerman,MR
朋友鼠红白血病细胞分化过程中蛋白质合成减少与细胞内Na的关系。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lannigan,DA;Knauf,PA;Macara,IG
通讯作者：
Macara,IG